Biodegradation of Anthracene: Influence Of selected physiochemical parameters and metabolism

L. Brinda, M. Velan
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引用次数: 1

Abstract

In the present study, Anthracene, a model PAH compound was preferred because of its high toxic and carcinogenic property. Biodegradation was premeditated with an isolated, characterized and acclimatized strain of Alcaligenes faecalis MVMB1 obtained from petroleum contaminated soil. The optimum parameters for maximum anthracene degradation and growth were observed at 2% inoculum concentration and 6.5 pH. Within 40 hrs and at 30°C, the strain metabolized 92.34 % of anthracene (40 mg l−1) under optimized conditions. Experimentally, it was observed that anthracene was inhibitory type substrate as the inhibition effect became predominant above the concentration of 40 mg l−1. Three degradation pathways were proposed based on the identified metabolites. A novel metabolite identified as 9 hydroxy fluorene demonstrates a new branch in anthracene degradation pathway via dihydroxyphthalate. These results indicate that Alcaligenes faecalis MVMB1 is effective in degrading anthracene and also proves its ability to degrade fluorene, another PAH compound.
蒽的生物降解:选定的理化参数和代谢的影响
在本研究中,典型的多环芳烃化合物蒽因其高毒性和致癌性而被首选。利用从石油污染土壤中分离、鉴定和驯化的粪碱性菌MVMB1进行生物降解预处理。在接种量为2%、ph为6.5的条件下,菌株对蒽的最大降解和生长效果最佳。在此条件下,菌株在30℃、40 h内的蒽代谢率为92.34% (40 mg l−1)。实验结果表明,蒽为抑菌型底物,在40 mg l−1浓度以上,其抑菌效果显著。根据鉴定的代谢物,提出了三种降解途径。一种新的代谢物被鉴定为9羟基芴,在邻苯二甲酸二氢酯降解蒽的途径中开辟了新的分支。这些结果表明,粪Alcaligenes faecalis MVMB1不仅能有效降解蒽,还能降解另一种多环芳烃化合物芴。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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