Bioethanol Production Based on Simultaneous Saccharification and Fermentation of Wheat Straw

2010 International Conference on Challenges in Environmental Science and Computer Engineering Pub Date : 2010-03-06 DOI:10.1109/CESCE.2010.191

Peng Luo, Zhong Liu

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引用次数: 4

Abstract

The potential of wheat straw as raw materials for ethanol production was investigated. Ethanol cooking at 190 ¿ for 60 min was adopted as pretreatment method for dilute sulfuric acid impregnated wheat straw. The pretreated wheat straw was used as substrate for the production of ethanol by simultaneous saccharification and fermentation (SSF) through orthogonal experiment design in the present study. The in¿uence of different parameters in SSF, such as reaction temperature, substrate concentration, initial medium pH and enzyme concentration, on the ethanol concentration and yield was investigated and the SSF conditions were optimized. The ranking, from high to low, of influential extent of the SSF affecting factors to ethanol concentration and yield was substrate concentration, enzyme loading, initial fermentation liquid pH value, reaction temperature, orderly. The optimum SSF condition was 35¿ for reaction temperature, 100 gl-1 for substrate concentration, 5.0 for initial fermentation liquid pH value, 30 FPUg-1 for enzyme loading. Under the optimum SSF condition, 23.1 gl-1 of the ethanol concentration and 68.2% of the ethanol yield were obtained respectively after 72 h.

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麦秸糖化发酵生产生物乙醇的研究

研究了麦秸作为乙醇生产原料的潜力。采用190℃乙醇蒸煮60 min的预处理方法对稀硫酸浸渍麦秸进行预处理。本研究以预处理后的麦秸为底物，通过正交试验设计，采用同步糖化发酵法生产乙醇。考察了反应温度、底物浓度、初始培养基pH和酶浓度等参数对乙醇浓度和产率的影响，并优化了反应条件。SSF影响因素对乙醇浓度和产率的影响程度由高到低依次为底物浓度、酶载量、发酵液初始pH值、反应温度。SSF的最佳条件为反应温度35℃，底物浓度100 gl-1，发酵液初始pH值5.0，酶载量30 fug -1。在最佳SSF条件下，72 h后乙醇浓度为23.1 g / 1，乙醇收率为68.2%。

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2010 International Conference on Challenges in Environmental Science and Computer Engineering

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