A novel sorbent based on metal–organic framework for mercury separation from human serum samples by ultrasound assisted- ionic liquid-solid phase microextraction
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引用次数: 17
Abstract
In this research, the metal–organic framework (MOF) as a solid phase was used for separation mercury [Hg (II)] inhuman serum sample by ultrasound assisted- Ionic Liquid-solid phase microextraction procedure (USA- IL-μ-SPE). Mercury extracted from serum sample by [Zn2(BDC)2(DABCO)]n as MOF at pH=7.8. Hydrophobic ionic liquid ([BMIM] [PF6]) was used as solvent trap for Hg-MOF-NC from the sample solution. The phase of Hg-MOF-NC was back extracted by 0.5 mL of HNO3 (0.2 mol L-1) and finally mercury concentration determined with cold vapor-atomic absorption spectrometry (CV-AAS) after dilution with 0.5 mL of DW. Under the optimal conditions, the linear range, limit of detection and preconcentration factor were obtained 0.02–5.5 µg L−1, 6.5 ng L−1 and 9.8 for serum samples, respectively (%RSD<5%). The validation of methodology was confirmed by standard reference materials (SRM).
本研究以金属有机骨架(MOF)为固相,采用超声辅助离子液-固相微萃取法(USA- IL-μ- spe)分离非人类血清样品中的汞[Hg (II)]。在pH=7.8时,用[Zn2(BDC)2(DABCO)]n作为MOF从血清样品中提取汞。疏水离子液体([BMIM] [PF6])作为样品溶液中Hg-MOF-NC的溶剂捕集剂。用0.5 mL HNO3 (0.2 mol L-1)反提Hg-MOF-NC相,用0.5 mL DW稀释后,用冷气相原子吸收光谱法测定汞浓度。在最佳条件下,血清样品的线性范围为0.02 ~ 5.5 μ g L−1,检出限为6.5 ng L−1,预富集因子为9.8 (%RSD<5%)。采用标准对照品(SRM)对方法进行验证。