Turbidimetric determination of orthophosphate in digested plant material by flow-injection analysis

Laboratory Robotics and Automation Pub Date : 2000-12-18 DOI:10.1002/1098-2728(2000)12:5<236::AID-LRA3>3.0.CO;2-L

Maria-Celeste T. Diniz, André Fernando Oliveira, Orlando Fatibello-Filho

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引用次数: 3

Abstract

A simple, fast, and low-cost flow-injection system is proposed for the determination of orthophosphate in digested plant material. The determination is based on the precipitation reaction of orthophosphate with 0.05 mol L⁻¹ zinc solution in 0.05 mol L⁻¹ triethanolamine buffer (TEA/TEAH⁺, pH 6.0) solution containing 0.025% (w/v) poly-(vinylalcohol). The zinc orthophosphate suspension produced was directly measured at 410 nm. Under the best experimental conditions, orthophosphate was determinated in the concentration range from 5 to 60 mg P L⁻¹, and the relative standard deviations were less than 1.6% for 10 and 20 mg P L⁻¹ solutions (n = 9). The analytical frequency was 180 h⁻¹. The results obtained using the proposed FIA procedure and those obtained using a standard spectrophotometric procedure agree at the 95% confidence level. © 2000 John Wiley & Sons, Inc. Lab Robotics and Automation 12:236–240, 2000

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流动注射分析法浊度法测定消化植物原料中正磷酸盐

提出了一种简单、快速、低成本的流动注射系统，用于测定消化植物材料中的正磷酸盐。在0.05 mol L−1的三乙醇胺缓冲液(TEA/TEAH+， pH 6.0)含0.025% (w/v)聚乙烯醇溶液中，正磷酸盐与0.05 mol L−1锌溶液沉淀反应。制备的正磷酸锌悬浮液在410 nm处直接测定。在最佳实验条件下，正磷酸盐的测定浓度范围为5 ~ 60 mg P L−1,10和20 mg P L−1溶液(n = 9)的相对标准偏差小于1.6%，分析频率为180 h−1。使用建议的FIA程序获得的结果和使用标准分光光度法程序获得的结果在95%的置信水平上一致。©2000 John Wiley &儿子,Inc。机械工程学报，2009 (12):356 - 356

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Laboratory Robotics and Automation

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