Influence of Various Culture Media on Growth and Carbohydrates Content in a Cyanobacterium Nostoc muscorum

Abstract

Method of collection: The soil samples from 5-10 cm deep soil layers were collected using the scalpels. Soil samples were collected in polythene bags of size 6 x 4 inches. ABSTRACT: Nostoc muscorum was isolated from the collected soil samples from different locations. Identification was carried out using morphological variation and taxonomical approaches according to Desikachary (1959). The axenic culture of Nostoc muscorum was obtained in the laboratory. For the biomass production, different culture media were used namely BG-11, Fogg’s medium, Allen and Arnon medium, Zarrouk’s medium and CFTRI medium. The biomass was harvested by filtration through double layered muslin cloth and dried using air blower. After harvesting, the biomass obtained was subjected to the growth analysis. The total carbohydrates were estimated by following Anthrone method (Hedge and Hofreiter, 1962). Out of the different culture media used, BG-11 medium supported the growth of Nostoc muscorum properly as compared to other media used. The total carbohydrates content was more in Nostoc muscorum grown in CFTRI medium followed by the Allen and Arnon medium. © 2019 iGlobal Research and Publishing Foundation. All