Application of NAD(P)H oxidase for cofactor regeneration in dehydrogenase catalyzed oxidations

Q2 Chemical Engineering

Journal of Molecular Catalysis B-enzymatic Pub Date : 2016-12-01 DOI:10.1016/j.molcatb.2016.09.016

Gustav Rehn, Asbjørn Toftgaard Pedersen, John M. Woodley

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引用次数: 44

Abstract

Biocatalytic oxidations can offer clear advantages compared to chemically catalyzed oxidations in terms of chemo, regio and stereoselectivity as well as a reduced environmental impact. One of the most industrially important reactions is the oxidation of alcohols, which can be carried out using alcohol dehydrogenases. However, their effective use requires an effective regeneration of the oxidized nicotinamide cofactor (NAD(P)⁺), which is critical for the economic feasibility of the process. NAD(P)H oxidase is an enzyme class of particular interest for this cofactor regeneration since it enables the use of molecular oxygen as a substrate, generating either water or hydrogen peroxide as a by-product. The use of these enzymes is now gaining an increased interest, and several different enzymes of both types have been applied for proof-of-concept. In this review, we give an overview of the state-of-the-art, and discuss several important issues for future implementation in a production process.

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NAD(P)H氧化酶在脱氢酶催化氧化中辅助因子再生的应用

与化学催化氧化相比，生物催化氧化在化学、区域和立体选择性以及减少对环境的影响方面具有明显的优势。工业上最重要的反应之一是醇的氧化，这可以用醇脱氢酶来进行。然而，它们的有效利用需要氧化烟酰胺辅助因子(NAD(P)+)的有效再生，这对该工艺的经济可行性至关重要。NAD(P)H氧化酶是这种辅助因子再生特别感兴趣的一类酶，因为它可以使用分子氧作为底物，产生水或过氧化氢作为副产物。现在对这些酶的使用越来越感兴趣，两种类型的几种不同的酶已被用于概念验证。在这篇综述中，我们概述了最新的技术，并讨论了未来在生产过程中实施的几个重要问题。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Molecular Catalysis B-enzymatic 生物-生化与分子生物学

CiteScore

2.58

自引率

0.00%

发文量

审稿时长

3.4 months

期刊介绍： Journal of Molecular Catalysis B: Enzymatic is an international forum for researchers and product developers in the applications of whole-cell and cell-free enzymes as catalysts in organic synthesis. Emphasis is on mechanistic and synthetic aspects of the biocatalytic transformation. Papers should report novel and significant advances in one or more of the following topics; Applied and fundamental studies of enzymes used for biocatalysis; Industrial applications of enzymatic processes, e.g. in fine chemical synthesis; Chemo-, regio- and enantioselective transformations; Screening for biocatalysts; Integration of biocatalytic and chemical steps in organic syntheses; Novel biocatalysts, e.g. enzymes from extremophiles and catalytic antibodies; Enzyme immobilization and stabilization, particularly in non-conventional media; Bioprocess engineering aspects, e.g. membrane bioreactors; Improvement of catalytic performance of enzymes, e.g. by protein engineering or chemical modification; Structural studies, including computer simulation, relating to substrate specificity and reaction selectivity; Biomimetic studies related to enzymatic transformations.