Xie Zhixun, Xie Liji, Pang Yaoshan, Liu Jiabo, Deng Xianwen, Xie Zhiqin
{"title":"Development of a duplex real-time PCR assay for detection of Perkinsus and Marteilia refringens in shellfish.","authors":"Xie Zhixun, Xie Liji, Pang Yaoshan, Liu Jiabo, Deng Xianwen, Xie Zhiqin","doi":"10.3724/SP.J.1035.2010.00898","DOIUrl":null,"url":null,"abstract":"[Objective] To improve the accuracy and efficiency of the detection which used in Perkinsus sp and Marteilia refringens, and then shorten the detective cycle.[Method]According to the gene sequence of Perkinsus sp and Marteilia refringens from gene bank, design two pairs of specific primers and two TaqMan probes with different fluorophores labeled. Optimizing the reactive conditions and reagent concentration in order that establishing the duplex real-time PCR method for detecting Perkinsus sp and Marteilia refringens simultaneously.[Result] The sensitivity of the duplex real-time PCR method which about Perkinsus sp and Marteilia refringens is 40 template copies. After combine the templates of Perkinsus sp and Marteilia refringens with different concentrations, this method still could be detect this two protozoan efficiently and synchronously.[Conclusion] The established duplex real-time PCR method for detecting Perkinsus sp. and Marteilia refringens possesses lots of advantages, such as specific, sensitive, rapid, quantitative and reproducible, can be used for clinical detection of infection which was caused by Perkinsus sp. and Marteilia refringens.","PeriodicalId":7791,"journal":{"name":"畜牧与饲料科学","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2010-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"畜牧与饲料科学","FirstCategoryId":"1091","ListUrlMain":"https://doi.org/10.3724/SP.J.1035.2010.00898","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
[Objective] To improve the accuracy and efficiency of the detection which used in Perkinsus sp and Marteilia refringens, and then shorten the detective cycle.[Method]According to the gene sequence of Perkinsus sp and Marteilia refringens from gene bank, design two pairs of specific primers and two TaqMan probes with different fluorophores labeled. Optimizing the reactive conditions and reagent concentration in order that establishing the duplex real-time PCR method for detecting Perkinsus sp and Marteilia refringens simultaneously.[Result] The sensitivity of the duplex real-time PCR method which about Perkinsus sp and Marteilia refringens is 40 template copies. After combine the templates of Perkinsus sp and Marteilia refringens with different concentrations, this method still could be detect this two protozoan efficiently and synchronously.[Conclusion] The established duplex real-time PCR method for detecting Perkinsus sp. and Marteilia refringens possesses lots of advantages, such as specific, sensitive, rapid, quantitative and reproducible, can be used for clinical detection of infection which was caused by Perkinsus sp. and Marteilia refringens.
期刊介绍:
Animal Husbandry and Feed Science is an academic journal supervised and sponsored by the Academy of Agricultural and Animal Husbandry Sciences of Inner Mongolia Autonomous Region. The journal was founded in 1973, the original name of the journal is “Inner Mongolia Animal Husbandry Science”, changed to the current name in 2003. Domestic uniform issue number: CN 15-1228/S; international standard issue number: ISSN 1672-5190; international journal code (CODEN): XYSKAX.
Animal Husbandry and Feed Science is one of the first academic journals recognized by the State Administration of Press, Publication, Radio, Film and Television (SARFT), and has been selected as one of China's scientific and technological core journals (2021 and 2022 editions), RCCSE China's Core Academic Journals (2017-2020), and China's Agricultural and Forestry Core Journals (2020 edition); It is included in the full text of domestic academic resource databases, such as Zhi.com, Wanfang, Wipu, Superstar, etc. The English abstracts of the articles published in the journal are included in the CAB Abstracts database under the Research Center for Agricultural and Biological Sciences of the United Kingdom (CABI) , and in the full text of the database of the U.S. EBSCO; and it is a source journal of the “Annual Report of Impact Factor of Chinese Academic Journals”.