A colorimetric method for the determination of different functional flavonoids using 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and peroxidase

F. R. Marín, J. Hernández-Ruiz, M. B. Arnao
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引用次数: 3

Abstract

Abstract In many occasions it is necessary to use fast and simple methods, different to the chromatographic techniques, for the quantification of biomolecules such as flavonoids. Also, the flavonoid levels in some foodstuffs can be influenced by industrial extraction processes such as pressing and squeezing, resulting in modification of their functional value. For this purpose, we have developed a rapid method to analyze flavonoids, based on a coupling reaction between ABTS and flavonoid mediated by peroxidase. The present method can be used to detect and measure flavonoids with hydroxyl moieties on A- or B-rings, not adjacent to methoxy or oxo substitutions. The visible spectrum of the ABTS-flavonoid complex, the calibration curve (within the range 5-50 μM) and the molar absorption coefficients for isosakuranetin, isonaringin, rhoifolin, hyperoside, rutin, hesperetin, quercetin, kaempherol and naringenin are given. The method has been applied to complex culture media and is sensitive, accurate, quick and easy to apply. This method can be used in laboratories that do not have sophisticated and expensive techniques such as liquid chromatography and also as a quick, simple and inexpensive technique for student practice laboratories.
用2,2′-氮基-双-(3-乙基苯并噻唑-6-磺酸)(ABTS)和过氧化物酶测定不同功能黄酮类化合物的比色法
摘要在许多情况下,需要采用不同于色谱技术的快速、简便的方法来定量黄酮类化合物等生物分子。此外,某些食品中的类黄酮水平可能受到工业提取过程(如压榨和挤压)的影响,从而导致其功能价值的改变。为此,我们建立了一种基于ABTS与类黄酮在过氧化物酶介导下的偶联反应的快速分析方法。本方法可用于检测A环或b环上不毗邻甲氧基或氧基取代的羟基部分的类黄酮。给出了abts -类黄酮配合物的可见光谱、5 ~ 50 μM范围内的标定曲线和对异紫金苷、异紫金苷、红花叶苷、金丝桃苷、芦丁、橙皮苷、槲皮素、山柰酚、柚皮素的摩尔吸收系数。该方法适用于复杂培养基,具有灵敏、准确、快速、简便等特点。该方法可用于没有液相色谱等复杂和昂贵技术的实验室,也可作为学生实践实验室快速,简单和廉价的技术。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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