Effect of Diabetes Mellitus and Hypertension on Osmotic Fragility and Hemorheological Factors in Male Wistar Rats

David Ehikhuemen Okonofua, J. N. Asiwe, K. K. Anachuna, E. G. Moke, K. Sanusi, Ebunoluwa Oluwabusola Adagbada, Mariam Onono Yusuf, Damilola Ifeoluwa Alawode, A. Fasanmade
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引用次数: 7

Abstract

Diabetes mellitus is a common risk factor for erythrocyte osmotic stress. This study was aimed at exploring the effect of streptozotocin (STZ)-induced diabetes mellitus and salt-induced hypertension on osmotic fragility and hemorheological variables in male Wistar rats. Thirty male rats were grouped into five groups of six animals each as follows: negative control (zero salt in diet); positive control (normal salt diet - 0.3% salt); high salt diet (8% salt) (HSD only); STZ induced diabetes and normal salt diet (STZ only); STZ induced diabetes and high salt diet (STZ + HSD). At the end of a 4 weeks period, hematological variables, osmotic fragility, rheology and cardiovascular responses were assessed. There was an increase (p<0.05) in the mean arterial pressure and heart rate of HSD, STZ and HSD + STZ groups indicating a salt induced hypertension. There was a decrease in the body weight of STZ and HSD +STZ groups. There was significant increase (p<0.05) in the haematocrit, platelets estimates and fibrinogen concentrations in the experimental groups when compared with the controls. The STZ and STZ + HSD groups showed a reduced clotting time which corresponded to the increased platelet estimates and fibrinogen concentration. The increase in haematocrit, platelet and plasma protein resulted in the increased blood viscosity and a decreased flow rate. The osmotic fragility test was also observed to be increased (p<0.05) in HSD, STZ only and STZ + HSD groups. Diabetes mellitus and hypertension increase the rate of hemolysis of erythrocyte, as well as increase blood viscosity.
糖尿病和高血压对雄性Wistar大鼠渗透脆性和血液流变学因子的影响
糖尿病是红细胞渗透应激的常见危险因素。本研究旨在探讨链脲佐菌素(STZ)诱导的糖尿病和盐致高血压对雄性Wistar大鼠渗透脆弱性和血液流变学指标的影响。将30只雄性大鼠分为5组,每组6只:阴性对照(饮食中不含盐);阳性对照(正常盐饮食- 0.3%盐);高盐饮食(8%盐)(仅HSD);STZ诱导的糖尿病和正常盐饮食(仅限STZ);STZ诱导糖尿病和高盐饮食(STZ + HSD)。在4周结束时,评估血液学变量、渗透脆弱性、流变学和心血管反应。HSD组、STZ组和HSD + STZ组平均动脉压和心率升高(p<0.05),提示盐致高血压。STZ组和HSD +STZ组大鼠体重下降。与对照组相比,实验组红细胞压积、血小板估计值和纤维蛋白原浓度均显著升高(p<0.05)。STZ组和STZ + HSD组凝血时间缩短,这与血小板数量和纤维蛋白原浓度增加相对应。红细胞压积、血小板和血浆蛋白的增加导致血液粘度增加和血流速率降低。HSD组、单纯STZ组和STZ + HSD组的渗透脆性试验也显著升高(p<0.05)。糖尿病和高血压使红细胞溶血率增加,血液黏稠度增加。
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