Comparison of Protein Extraction Methods from Seeds of Three Opuntia (Cactaceae) Species

IF 2 4区生物学 Q2 PLANT SCIENCES

Haseltonia Pub Date : 2021-03-01 DOI:10.2985/026.027.0108

David I. Warren, Peter H. Quach, Anne R. Gaillard

引用次数: 0

Abstract

Abstract: Cactus seeds have unusual properties that resist protein extraction, e.g. a thick testa (seed coat) and a high amount of mucilage within the cells. This study was performed to determine which method (phenol extraction, TCA/acetone extraction, or phosphate-TCA/acetone extraction) is optimal for extracting protein from seeds of three species of Opuntia: Opuntia robusta, Opuntia phaeacantha, and Opuntia engelmannii. Extracted protein was analyzed using a Bradford assay and SDS-PAGE with silver staining. Overall results revealed that the TCA/acetone and phosphate-TCA/acetone methods were far more effective at extracting protein compared to the phenol extraction method. These protein extraction results can be applied to future studies of proteins important for Opuntia seed germination and conservation.

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三种仙人掌属植物种子蛋白质提取方法的比较

摘要:仙人掌种子具有抵抗蛋白质提取的特殊特性，如厚的种皮和细胞内大量的粘液。本研究确定了苯酚提取、TCA/丙酮提取或磷酸盐-TCA/丙酮提取三种欧米加(Opuntia robusta)、菲米加(Opuntia phaeacantha)和恩格曼欧米加(Opuntia engelmannii)种子蛋白质的最佳提取方法。提取的蛋白用Bradford法和SDS-PAGE银染色进行分析。结果表明，TCA/丙酮法和磷酸盐-TCA/丙酮法对蛋白质的提取效果明显优于苯酚法。这些蛋白质的提取结果可以应用于未来对黑麦种子萌发和保存重要蛋白质的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Haseltonia 生物-植物科学

CiteScore

3.00

自引率

6.70%

发文量

审稿时长

>12 weeks

期刊介绍： Haseltonia, Yearbook of the Cactus and Succulent Society of America, is published in full color and features peer-reviewed articles about all aspects of cacti, succulents and their environs. Topics include current research and conservation reports, new species descriptions and lengthy taxonomic revisions, historical and biographical notes, chemical and cytological studies, evolutionary biology and ethnobotanical reports, propagation and pest control methods, and pollinator studies. Serious students of the world''s succulent flora, botanists, taxonomists, researchers and horticulturalists will all find Haseltonia a valuable addition to their book collection.