The effect of repeated freeze-thaw cycles on cryopreserved Neurospora crassa samples.

K. McCluskey, A. Wiest, S. Walker
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引用次数: 5

Abstract

To better characterize handling parameters for the arrayed mutants prepared for the Neurospora functional genomics program, we have put 7 day old conidia from strain FGSC 2489 through a series of cycles of freezing at -80 C in 25% glycerol and 3.5% reconstituted non-fat dry milk. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This brief note is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol53/iss1/12
反复冻融循环对粗神经孢子虫样品冷冻保存的影响。
为了更好地表征为神经孢子菌功能基因组学计划准备的排列突变体的处理参数,我们将菌株FGSC 2489的7天龄分生孢子放入25%甘油和3.5%重组脱脂干牛奶中,在-80℃下进行一系列冷冻循环。本作品采用知识共享署名-相同方式共享4.0许可协议。这个简短的说明可在真菌遗传学报告:http://newprairiepress.org/fgr/vol53/iss1/12
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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