Evaluation of Phytochemical Contents and Biological Activities of Salvia officinalis and Salvia triloba Grown with Organic Farming

Abstract

Salvia officinalis L., known as medicinal sage, and Salvia triloba L., known as Anatolian sage, belong to the Lamiaceae family and are species that usually grow in the Mediterranean region. In this study, it was aimed to evaluate the in vitro antidiabetic, antiobesity and antioxidant potentials of the extracts prepared by infusion technique from S. officinalis and S. triloba grown by organic farming methods. In addition, the effects of the extracts on the pancreatic cholesterol esterase enzyme were also investigated. Reverse Phase-HPLC technique was used to analyze the phytochemical contents of the extracts. At a concentration of 2 mg/mL, S. officinalis inhibited 64.69% ± 0.23, S. triloba 47.78 ± 2.11% on the α-glucosidase enzyme. Only S. triloba had an inhibitory effect on α-amylase and pancreatic lipase enzyme. On the pancreatic cholesterol esterase enzyme, inhibition values of S. triloba extract at all tested concentrations were found to be higher than S. officinalis extract. When the antioxidant potentials of the extracts were evaluated, the reducing power absorbance values were found to be the highest of the S. officinalis extract. The metal chelating capacity of both extracts at a concentration of 2 mg/mL was calculated as 100%. It was concluded that the ABTS radical scavenging activity of the extracts increased in a dose-dependent manner. With the Reverse Phase-YPSK technique, rosmarinic acid and hesperidin were found to be higher in S. officinalis extract. The presence of hesperidin in S. triloba was detected for the first time in this study. Considering all these findings, it was concluded that activity-guided isolation and in vivo activity studies should be performed because these two species grown by organic farming method have strong α-glucosidase enzyme inhibitory and antioxidant effects.