Medicinal prospective of Citrus limon and Citrus sinensis peels essential oil by Gas Chromatography/ Mass Spectrometry (GC/MS) compositional analysis

C. Edenta, P. E. Ozioma, C. J. Okoye, V. Nwankwo, O. Uraku, A. Uraku, C. Ezeali
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Abstract

Citrus fruits are highly nutritious and medicinal fruits that are commonly cultivated throughout the tropics. This study was carried out to determine the bioactive compounds of the methanol peel extracts of Citrus limon and Citrus sinensis. The peels from fresh fruits of C. limon and C. sinensis were air-dried at room temperature and were pulverized into fine powder. The bioactive determinations were carried out by Gas Chromatography/Mass Spectrometry (GC/MS). The mass spectrum of the compounds found in the peels extract was matched with the National Institute of Standards and Technology (NIST) library. The results showed that four (4) and five (5) bioactive compounds were identified in C. limon and C. sinensis methanol peel extracts respectively. The C. limon peel extract contained 4-(methylsulfanyl) but-1-en-3-yne, Sulfanylphenylium, 1,3-thiazole and 3-methylidenecyclobutanecarbonitrile while C. sinensis contained Pyridine-2-carbaldehyde, 1-Methyl-1H-pyrrole-2-carbaldehyde, Glutamic acid, 2-Ethyl-5-methyl-1H-pyrrole and Pyrrolidin-2-one. The results of this study therefore unveiled that the C. limon and C. sinensis peel extracts contained variable bioactive compounds with medicinal value and could be useful raw materials for pharmaceutical industries.
气相色谱/质谱联用分析柠檬和柑橘果皮精油的药用前景
柑橘类水果是营养丰富的药用水果,在热带地区普遍种植。本研究对柠檬和柑桔甲醇皮提取物的生物活性成分进行了测定。在室温下,将新鲜柠檬果皮和柑橘果皮风干,粉碎成细粉。生物活性测定采用气相色谱/质谱联用(GC/MS)技术。在果皮提取物中发现的化合物的质谱与美国国家标准与技术研究所(NIST)文库相匹配。结果表明,从柠檬柑甲醇皮提取物中分别鉴定出4种和5种活性化合物。柠檬皮提取物中含有4-(甲基磺胺基)-1-烯-3-炔、磺胺基苯基、1,3-噻唑和3-甲基环丁腈,而柠檬皮提取物中含有吡啶-2-醛、1-甲基- 1h -吡咯-2-醛、谷氨酸、2-乙基-5-甲基- 1h -吡咯和吡咯-2-酮。本研究结果表明,柠檬皮和柑橘皮提取物含有多种具有药用价值的生物活性化合物,可作为制药工业的重要原料。
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