A Highly Convenient Procedure for Oligodeoxynucleotide Purification

Abstract

Purification of synthetic oligodeoxynucleotides (ODNs) is simply achieved by capping failure sequences with a polymerizable phosphoramidite followed by polymerization. In this article, the reduction of the amount of polymerization monomer to drastically increase ODN extraction efficiency, the use of a centrifugal filterunit to ease the extraction process and the notification of using fresh phosphoramidite solutions are described. In addition, further evidence to support the purity of ODN and discussions of ODN stability under radical polymerization conditions are provided.