Effects of Lead (Pb) and Cadmium (Cd) Elements on Lipid Peroxidation, Catalase Enzyme Activity and Catalase Gene Expression Profile in Tomato Plants

4区农林科学

Tarim Bilimleri Dergisi-journal of Agricultural Sciences Pub Date : 2016-12-12 DOI:10.1501/TARIMBIL_0000001412

Semra Aydin, I. Büyük, Esra Gündüzer, Burcu Pelin Büyük, I. Kandemir, Demet Duman, S. Aras

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引用次数: 8

Abstract

Heavy metals are significant abiotic stress factor, affecting various response mechanisms in plants. These responses include: changes in membrane composition, production of small molecules and free radicals, and alterations in the activities of antioxidant enzymes and their gene expressions. For this reason, lipid peroxidation levels (MDA), catalase enzyme activity, and gene expression profiles, quantified by real-time PCR, were analyzed in tomato plants exposed to various concentrations (0, 80, 160, 320, 640 and 1280 μM) of Cd2+ and Pb2+. All concentration of Cd+2 or Pb+2 contamination led to increased lipid peroxidation and catalase enzyme activity, except for 320 and 640 μM Cd+2 contamination levels. As a result, gene expression patterns at the mRNA level and changes in MDA content under different concentrations of Pb+2 and Cd+2 contamination revealed a positive correlation, although no correlation was found between gene expression patterns at the mRNA level and catalase enzyme activity. These results might be explained by the regulation of genes at the transcriptional, posttranscriptional, and also translational or posttranslational levels.

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铅、镉元素对番茄脂质过氧化、过氧化氢酶活性及过氧化氢酶基因表达谱的影响

重金属是重要的非生物胁迫因子，影响植物的多种生理反应机制。这些反应包括:膜组成的变化，小分子和自由基的产生，以及抗氧化酶活性及其基因表达的变化。为此，利用实时荧光定量PCR技术，对暴露于不同浓度(0、80、160、320、640和1280 μM) Cd2+和Pb2+的番茄植株进行了脂质过氧化水平(MDA)、过氧化氢酶活性和基因表达谱分析。除浓度为320 μM和640 μM的Cd+2污染外，所有浓度的Cd+2和Pb+2污染均导致脂质过氧化和过氧化氢酶活性升高。结果表明，不同浓度Pb+2和Cd+2污染下，mRNA水平上的基因表达模式与MDA含量的变化呈正相关，而mRNA水平上的基因表达模式与过氧化氢酶活性无相关性。这些结果可以用基因在转录、转录后、翻译或翻译后水平的调控来解释。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tarim Bilimleri Dergisi-journal of Agricultural Sciences 农林科学-农业综合

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Journal of Agricultural Sciences (JAS) is an international, double-blind peer-reviewed, open-access journal, published by the Faculty of Agriculture, Ankara University. The journal invites original research papers containing new insight into any aspect of Agricultural Sciences that are not published or not being considered for publication elsewhere. Preliminary, confirmatory or inconclusive research, review articles, case and local studies and works presenting taxonomy will not be published.