Evaluation of analgesic activity of ethanolic and aqueous extracts of leaf of Plumeria rubra in albino rat

Dhanapal Venkatachalam, B. Thavamani, Muddukrishniah
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引用次数: 2

Abstract

Objective : Plumeria rubra is an important traditional medicinal plant used in various ailments and the indigenous plant as a medicament for treatment of various inflammatory conditions is well documented in literature. The analgesic activity was evaluated by hot-plate and acetic acid induced writhing methods. Methods : The leaves of P. rubra were collected from the local area in and around Palakkad, Kerala (India). About 1500 g of the powder was extracted using Soxhlet apparatus for 12 h using 5.0 L of Ethanol as solvent. The test extracts was administered intraperitoneally at a dose of 100 and 200 mg/kg while Pentazocine (5 mg/kg) and diclofenac sodium (10 mg/kg) served as standards. Results : The ethanolic extract of 200 mg/kg having significant analgesic activity in Acetic acid induced abdominal writhing response and percentage of inhibition (PI) (p<0.01 )when compared to control.. The Ethanolic extract of 100 mg/kg and 200 mg/kg exhibited significant analgesic activity in the hot plate method (p<0.001) by increasing the pain reaction time (PAT) of the rats to  sec 7.25±0.38 at 45 min after treatment in comparison to control (4.08±13). Conclusions : The results suggest that ethanolic extracts of P. rubra possesses potent analgesic properties, which support its use in traditional medicine and suggesting that the plant should be further investigated for its pharmacological active natural products.
白化大鼠黄花鸡蛋花叶乙醇提取物及水提物的镇痛作用评价
目的:红鸡蛋花是一种重要的传统药用植物,用于治疗各种疾病,其作为治疗各种炎症的药物已有文献记载。采用热板法和醋酸扭体法评价其镇痛活性。方法:在印度喀拉拉邦Palakkad及周边地区采集红叶。以5.0 L乙醇为溶剂,用索氏仪提取约1500 g粉末,提取12 h。实验提取液分别以100和200 mg/kg的剂量腹腔注射,并以Pentazocine (5 mg/kg)和双氯芬酸钠(10 mg/kg)为标准。结果:与对照组相比,200 mg/kg乙醇提取物对醋酸诱导的大鼠扭腹反应和抑制率(PI)均有显著的镇痛作用(p<0.01)。100 mg/kg和200 mg/kg乙醇提取物在热板法中表现出显著的镇痛活性(p<0.001),治疗后45 min大鼠的疼痛反应时间(PAT)为7.25±0.38秒,对照组为4.08±13秒。结论:红颜草乙醇提取物具有较强的镇痛作用,支持其在传统医学中的应用,建议对其活性天然产物进行进一步的药理研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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