Amplification of cytochrome C oxidase summit I gene of Brandt's Vole by nested PCR.

Q3 Medicine

中华地方病学杂志 Pub Date : 2015-01-20 DOI:10.3760/CMA.J.ISSN.2095-4255.2015.01.018

Ma Zhidong, Chang Zili, L. Fang, Huo Yanhong, Lin Jianyun, Wu Zhenghua, Zhang Zhongbing

引用次数: 0

Abstract

Objective To determine a method for amplification of cytochrome C oxidase subunit I(CO I) gene of Brandt's vole. Methods The Brandt's Voles were captured in Abagaqi Xilingol League Inner Mongolia, and DNA was extracted from liver tissue. CO I gene was amplified by nested PCR and sequenced afterwards. Results A band of 657 bp and 1 132 bp was amplified by internal and external PCR primers, respectively, which were consistent with expected sizes. A total of 12 segments of Brandt's Vole CO I gene sequences were amplified by PCR and verified by sequencing. The sequence number was KF182196 - KF182207 in GenBank. After gene sequence alignment of the 12 CO I gene sequences, it was found that the similarity was 100%, and no base mutation. Conclusion CO I gene of Brandt's Vole could be amplified by nested PCR without pseudo gene. Key words: Nested PCR; Brandt's Vole; Cytochrome C oxidase subunit I gene

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巢式PCR扩增布氏田鼠细胞色素C氧化酶峰1基因。

目的建立一种扩增布氏田鼠细胞色素C氧化酶I亚基(coi)基因的方法。方法在内蒙古锡林郭勒盟阿巴嘎齐捕获布氏田鼠，提取肝组织DNA。采用巢式PCR扩增CO 1基因，并进行测序。结果内部引物和外部引物扩增出的条带长度分别为657 bp和1 132 bp，与预期大小一致。通过PCR扩增出勃兰特田鼠CO I基因序列共12段，并进行测序验证。序列编号为KF182196 - KF182207。对12个CO I基因序列进行基因序列比对，发现相似性为100%，无碱基突变。结论不带伪基因的巢式PCR法可以扩增出布氏田鼠CO 1基因。关键词:巢式PCR;布氏田鼠;细胞色素C氧化酶亚基I基因

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来源期刊

中华地方病学杂志我国对人类健康危害特别严重的地方性疾病：克山病、大骨节病、碘缺乏病、地方性氟中毒、地方性砷中毒、鼠疫、布鲁氏菌病、寄生虫、新冠肺炎等疾病，同时还报道多发性自然疫源性疾病。

CiteScore

1.60

自引率

0.00%

发文量

8714

期刊介绍： The Chinese Journal of Endemiology covers predominantly endemic diseases threatening health of the people in the areas affected by the diseases including Keshan disease, Kaschin-Beck Disease, iodine deficiency disorders, endemic fluorosis, endemic arsenism, plague, epidemic hemorrhagic fever, brucellosis, parasite diseases and the diseases related to local natural and socioeconomic conditions; and reports researches in the basic science, etiology, epidemiology, clinical practice, control as well as multidisciplinary studies on the diseases.