Optimization and production kinetics for cellulases by wild and mutant strain of Thermomyces dupontii in stirred tank reactor

IF 0.6 4区综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES

Acta Scientiarum-technology Pub Date : 2022-03-11 DOI:10.4025/actascitechnol.v44i1.57664

Kinza Nisar, Roheena Abullah, A. Kaleem, M. Iqtedar, Tehreema Iftikar

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引用次数: 1

Abstract

The main bottleneck in fermentation technology is scaling up procedure of industrial enzymes according to the biological characteristics of the organism. The current study describes the production kinetics of cellulases in stirred tank reactor by using mutant and wild strains of T. dupontii. The fermentation span of both the strains in bioreactor was examined. It is was found in mutant strain of T. dupontii fermentation time required for optimum production was reduced to 48h as compared to 72h in wild strain. The kinetic studies also exhibited greater value of µ (h-1) in case of mutated strain in comparison with wild strain. The effects of some other critical factors like agitation intensity dissolve oxygen, aeration, temperature, size of inoculum and pH was estimated on enzyme production kinetics. The results shows maximum activity of cellulases was attained at 220 rpm, 15% dissolve oxygen, aeration rate 1.5 vvm, 55°C, 8 % inoculum size and pH 5.5 for both strains respectively. The higher values of enzyme production kinetics i.e. Yp/x, Qp, Qx and qp in STR in case of mutant strain indicates its superiority over wild strain of T. dupontii. Thus mutant thermophilic T. dupontii might be a potential candidate for industrial applications.

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杜氏温氏菌野生和突变菌株在搅拌槽反应器中纤维素酶的优化和生产动力学

发酵技术的主要瓶颈是根据生物的生物学特性来扩大工业酶的生产规模。本文研究了杜氏体突变菌株和野生菌株在搅拌釜反应器中生产纤维素酶的动力学。研究了两种菌株在生物反应器中的发酵时间。结果表明，突变菌株的最佳产量发酵时间由野生菌株的72h缩短至48h。动力学研究还显示，突变菌株的µ(h-1)值比野生菌株大。考察了搅拌强度、溶解氧、曝气、温度、接种量大小和pH等关键因素对产酶动力学的影响。结果表明，在220转/分、溶解氧15%、曝气率1.5 vvm、55℃、接种量8%、pH 5.5条件下，两菌株的纤维素酶活性最高。突变菌株STR的产酶动力学Yp/x、Qp、Qx和Qp值较高，表明其优于野生菌株。因此，突变型嗜热菌可能是工业应用的潜在候选者。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Scientiarum-technology 综合性期刊-综合性期刊

CiteScore

1.40

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： The journal publishes original articles in all areas of Technology, including: Engineerings, Physics, Chemistry, Mathematics, Statistics, Geosciences and Computation Sciences. To establish the public inscription of knowledge and its preservation; To publish results of research comprising ideas and new scientific suggestions; To publicize worldwide information and knowledge produced by the scientific community; To speech the process of scientific communication in Technology.