Prevalence of bacteriocinogenic Rhizobium spp. in mungbean (Vigna radiata)

IF 0.8 4区生物学 Q3 AGRONOMY

Legume Research Pub Date : 2018-05-05 DOI:10.18805/LR-3884

P. K. Maan, S. Garcha, G. S. Walia

引用次数: 3

Abstract

The present work was undertaken to ascertain prevalence of bacteriocinogenic Rhizobium spp. in mungbean (Vigna radiata). Samples of rhizospheric soil and nodules were plated onto CRYEMA medium, selective for Rhizobium spp. From among the isolates obtained only four, designated as-N8, S1, S6, and S13 demonstrated bacteriocin production. Upto 104 AU/ml (Arbitrary Units/ml) of partially purified bacteriocin (PPB) was detected from N8 and S6. PPB obtained from N8 and S13 exhibited antagonism against indicator strain over a wide range of pH. All isolates of PPB were thermo-stable after heating to 90oC for 5 minutes. N8 exhibited 14.2mm zone of inhibition after heating to 50oC for 10 min. It decreased by 29.5% to 10mm (80oC/ 10min). S6 exhibited zone of inhibition of 11mm (50oC/10min) which decreased by 18.1% to 9mm (80oC/10min). Dual inoculation of nitrogen fixing Rhizobium with bacteriocin producing Rhizobium can ensure greater benefit than using nitrogen fixer alone.

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绿豆(Vigna radiata)中生菌根瘤菌的流行

本研究旨在确定绿豆(Vigna radiata)中致病菌根瘤菌的流行情况。根际土壤和根瘤样品被放置在对根瘤菌有选择性的CRYEMA培养基上，从分离得到的菌株中，只有4株(指定为n8、S1、S6和S13)显示出细菌素的产生。从N8和S6中检测到部分纯化的细菌素(PPB)最高可达104 AU/ml(任意单位/ml)。从N8和S13中获得的PPB在很宽的ph范围内对指示菌株表现出拮抗作用。所有PPB分离株在加热至90℃5分钟后都具有热稳定性。N8在50℃加热10min后呈现14.2mm的抑制区，在10mm(80℃/ 10min)时减小29.5%。S6表现出11mm (50oC/10min)的抑制区，减少了18.1%至9mm (80oC/10min)。固氮根瘤菌与产菌素根瘤菌双接种比单独使用固氮剂效果更好。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Legume Research AGRONOMY-

CiteScore

1.30

自引率

37.50%

发文量

256

审稿时长

6-12 weeks

期刊介绍： Legume Research An International Journal. The Journal is an official publication of Agricultural Research Communication Centre. It is designed to bring out the original research articles on genetics, breeding, physiology, bacterial avtivity, production, quality, biochemistry and seeds of legumes crops. The objective of the journal is to serve as a forum for scientific community to publish their research findings on legumes ans to provide basis for new research. Journal is being scanned in the important indexing and abstracting services.