Laura L. Eiberger, Carol L. Ventola, Bruce P. Wasserman
{"title":"Solubilization of a digitonin-stable glucan synthase from red beet root","authors":"Laura L. Eiberger, Carol L. Ventola, Bruce P. Wasserman","doi":"10.1016/0304-4211(85)90003-3","DOIUrl":null,"url":null,"abstract":"<div><p>UDP-glucose: 1,3-1,4 ß-glucan synthase was solubilized from red beet (<em>Beta vulgaris</em> L.) root microsomes by treatment with 1% digitonin at 30°C. The enzyme shows unusual stability for a solubilized glucan synthase, with full activity retention after storage for 1 week at 4°C.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(85)90003-3","citationCount":"13","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Science Letters","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0304421185900033","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 13
Abstract
UDP-glucose: 1,3-1,4 ß-glucan synthase was solubilized from red beet (Beta vulgaris L.) root microsomes by treatment with 1% digitonin at 30°C. The enzyme shows unusual stability for a solubilized glucan synthase, with full activity retention after storage for 1 week at 4°C.
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