Long-range heteronuclear spin locking (HSL) and its application to peptide sequencing

Abstract

In this paper, the utility of heteronuclear-spin-locking 2D NMR experiments for structure elucidation from long-range interactions is demonstrated. Long-range {¹H}¹³C heteronuclear-shift-correlation spectra via spin locking provides peptide-sequencing information for gramicidin-S with considerably better sensitivity than was previously obtained with other long-range shift-correlation methods such as HOESY and COLOC.