UV –Visible Spectroscopy Method Development and Its Validation for the Analysis of Marketed Hair Dyes for Amine Content

Abstract

To develop a simple rapid, accurate and spectrophotometric reproducible method were developed for estimation of PARA - PHENYLENE DIAMINE in different Marketed  Hair dyes . The analysis of PPD    was performed using NaOH   solution as diluent and using folins reagent at 432nm respectively .The methods were linear in the concentration range from 0-50µg/ml. The methods were validated with respect to system suitability, linearity, precision, limit of detection, limit of quantification, accuracy, ruggedness and robustness . The developed method can be used for routine analysis of   PARA PHENYLENE DIAMINE in marketed hair products. The methods were validated in accordance to the ICH guidelines.