Interrelation of IL1B and IL6 genes polymorphism with blood eosinophilia and histological picture of polyps in patients with chronic polypous rhinosinusitis

O. Y. Mezentseva, V. S. Piskunov, A. Polonikov
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引用次数: 1

Abstract

Background: The problem of chronic polyposis rhinosinusitis is one of the most common diseases in modern otorhinolaryngology with an etiopathogenesis that is not fully understood. A widely studied theory of nasal polyposis formation today is genetic. It is believed that some candidate genes, such as cytokine genes, can influence not only the onset of the disease itself, but also its clinical manifestations, such as blood eosinophilia and the histological structure of polyposis tissue, since this pathology is often accompanied by atopy with an increase in the level of eosinophils. The aim of the study: To study the associations of gene polymorphism IL1B (rs1143627) and IL6 (rs1800796) with blood eosinophilia and histological types of polyps in patients with chronic polypous rhinosinusitis. Materials and methods: An objective examination of racially and ethnically indistinguishable patients with nasal polyposis aged 18 to 60 years was carried out. The study involved 151 (37%) women and 257 (63%) men. A questionnaire was carried out with an emphasis on the socio-biological status of the patient, complaints, anamnesis of life and disease, data of clinical and laboratory examinations, features of the course of the disease. After venous blood was collected from the ulnar vein by standard phenol-chloroform extraction, DNA was isolated and then rs1143627 and rs1800796 genotyped. The odds ratio (OR) and 95% confidence interval (95% CI) were used to assess the presence of genotype associations; the correspondence of the distribution of genotype frequencies to the Hardy-Weinberg Оригинальная статья Original article Научные результаты биомедицинских исследований. 2021;7(4:363-374 Research Results in Biomedicine. 2021:7(4):363-374 365 equilibrium was judged by the p-level of significance. Results: An association of carriage of the G/G genotype (OR=0.31; 95 CI 0.10-0.92; p=0.037) of the IL1B gene (rs1143627) with a reduced probability of the formation of glandular polyps was revealed. No associations of polymorphic variants of the studied genes with blood eosinophilia were found. No functional effects of rs1143627 and rs1800796 in the tissues of the upper respiratory tract, nasal polyps and paranasal sinuses were found. The absence of the effect of rs1800796 on the expression of the IL6 gene was revealed. Conclusion: Thus, the revealed relationship of the G/G polymorphism of the IL1B gene (rs1143627) with a reduced likelihood of the formation of glandular polyps in patients with chronic polyposis rhinosinusitis may be evidence of the genetic causation of this disease, however, this hypothesis requires further study.
慢性息肉性鼻窦炎患者il - 1b和il - 6基因多态性与嗜酸性粒细胞增多和息肉组织学表现的关系
背景:慢性息肉病性鼻窦炎是现代耳鼻喉科最常见的疾病之一,其发病机制尚不完全清楚。目前广泛研究的鼻息肉形成理论是遗传的。据认为,一些候选基因,如细胞因子基因,不仅可以影响疾病本身的发病,还可以影响其临床表现,如血液嗜酸性粒细胞增多和息肉组织的组织学结构,因为这种病理常伴有嗜酸性粒细胞水平升高的特应性。研究目的:研究IL1B (rs1143627)和IL6 (rs1800796)基因多态性与慢性息肉性鼻窦炎患者血嗜酸性粒细胞增多和息肉组织学类型的关系。材料与方法:对18 ~ 60岁的鼻息肉患者进行了客观的人种和民族区分。该研究涉及151名(37%)女性和257名(63%)男性。进行了问卷调查,重点是患者的社会生物学状况、投诉、生活和疾病的记忆、临床和实验室检查数据、疾病过程的特征。标准苯酚-氯仿提取尺静脉静脉血,分离DNA,进行rs1143627和rs1800796基因分型。使用优势比(OR)和95%置信区间(95% CI)来评估基因型关联的存在;对应的基因型分布频率的哈迪温伯格Оригинальнаястатья原文Научныерезультатыбиомедицинскихисследований。2021:7(4):363-374生物医学研究成果,2021:7(4):363-374 365均衡通过p水平显著性判断。结果:携带G/G基因型的相关性(OR=0.31;95 ci 0.10-0.92;p=0.037)的IL1B基因(rs1143627)与腺体息肉形成的概率降低有关。未发现所研究基因的多态性变异与血嗜酸性粒细胞增多症有关。rs1143627和rs1800796在上呼吸道、鼻息肉和鼻窦组织中未发现功能影响。结果表明,rs1800796不影响il - 6基因的表达。结论:il - 1b基因(rs1143627) G/G多态性与慢性息肉病性鼻窦炎患者腺体息肉形成可能性降低的关系可能是该病遗传原因的证据,但这一假设有待进一步研究。
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