Chromosome Mapping of Repetitive DNAs in the Picasso Triggerfish (Rhinecanthus aculeatus (Linnaeus, 1758)) in Family Balistidae by Classical and Molecular Cytogenetic Techniques

IF 2.1 4区生物学 Q2 Agricultural and Biological Sciences

Caryologia Pub Date : 2023-02-12 DOI:10.36253/caryologia-1731

Kamika Sribenja, A. Tanomtong, N. Getlekha

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引用次数: 0

Abstract

This work presents the cytogenetic analysis conducted on the Picasso triggerfish (Rhinecanthus aculeatus (Linnaeus, 1758)) from Thailand. Mitotic chromosomes were prepared from the anterior kidney. The cell suspensions were harvested by in vivo colchicine treatment. The present study includes the chromosomal investigation on R. aculeatus, using conventional (Giemsa staining, Ag-NOR and C-banding) and molecular approaches (in situ mapping of five different repetitive DNA classes including 18S rDNA, 5S rDNA, (CA)15, (GA)15 and (CAA)10 as markers.) The results showed that R. aculeatus has karyotypes formed exclusively by telocentric chromosomes (44t; NF=44). The C-positive heterochromatic blocks are preferentially located in the centromeric and telomeric regions of some chromosomal pairs. The Ag-NOR sites occupy the interstitial position of the long arms of the largest telocentric pair (pair 1). The exclusive location of the major ribosomal sites in these pairs was confirmed by hybridization with 18S rDNA probes. However, the 5S rDNA genes are not located on 18S rDNA-bearing chromosomes, but instead located exclusively in the subcentromeric region of pair 4. The mapping of (CA)15, (GA)15 and (CAA)10 microsatellites are sparsely dispersed along all the chromosomes. The karyotype formula of R. aculeatus is 2n (44) = 44t.

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用经典和分子细胞遗传学技术定位鱼科毕加索触发鱼(Rhinecanthus aculeatus (Linnaeus, 1758))重复dna

本文介绍了对泰国毕加索触发器鱼(Rhinecanthus aculeatus (Linnaeus, 1758))进行的细胞遗传学分析。从前肾制备有丝分裂染色体。通过体内秋水仙碱处理获得细胞悬液。本研究采用常规方法(Giemsa染色、Ag-NOR和c带)和分子方法(原位定位包括18S rDNA、5S rDNA、(CA)15、(GA)15和(CAA)10等5种不同的重复DNA类别作为标记)对棘孔鲑进行染色体研究。结果表明，梭鲈的核型完全由远心染色体组成(44t;NF = 44)。c阳性异色区优先位于一些染色体对的着丝粒区和端粒区。Ag-NOR位点位于最大的远心对(对1)长臂的间隙位置。通过18S rDNA探针杂交证实了这些对中主要核糖体位点的排他位置。然而，5S rDNA基因并不位于携带18S rDNA的染色体上，而是完全位于第4对的亚着丝粒区。(CA)15、(GA)15和(CAA)10微卫星在所有染色体上稀疏分布。毛蚶核型公式为2n (44) = 44t。

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来源期刊

Caryologia 生物-遗传学

CiteScore

1.60

自引率

23.80%

发文量

审稿时长

12 months

期刊介绍： Caryologia is devoted to the publication of original papers, and occasionally of reviews, about plant, animal and human karyological, cytological, cytogenetic, embryological and ultrastructural studies. Articles about the structure, the organization and the biological events relating to DNA and chromatin organization in eukaryotic cells are considered. Caryologia has a strong tradition in plant and animal cytosystematics and in cytotoxicology. Bioinformatics articles may be considered, but only if they have an emphasis on the relationship between the nucleus and cytoplasm and/or the structural organization of the eukaryotic cell.