Efficacy of Qingfei oral liquid for idiopathic pulmonary fibrosis in rats and related network pharmacology study.

Yiwen Zhang, Kongsheng Sheng, Feifeng Song, Zongfu Pan, Xiaozhou Zou, Yujia Liu, Ping Huang
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Abstract

To investigate the therapeutic effect and mechanism of Qingfei oral liquid in idiopathic pulmonary fibrosis. Seventy-two male SD rats were divided into control group, model group, pirofenidone group and Qingfei group with 18 animals in each group. The idiopathic pulmonary fibrosis was induced in last three groups by intratracheal injection of bleomycin; pirofenidone group was given oral administration of pirofenidone b.i.d for 21 d, and Qingfei group was given Qingfei oral liquid 3.6 mL/kg q.d for Lung tissues were obtained for HE staining, Masson staining and transforming growth factor (TGF)-β immunohistochemical staining. Superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione (GSH) were detected in tissue homogenates. The BATMAN-TCM database was used to retrieve the chemical components and their corresponding targets of Qingfei oral solution by network pharmacology method, and then the component-target-disease network diagram was constructed. Finally, the pathway enrichment analysis was carried out to explore the molecular mechanism of Qingfei oral liquid against idiopathic fibrosis. Histopathology results showed that Qingfei oral liquid had a similar relieving effect on pulmonary fibrosis as the positive drug pirfenidone; TGF-β secretion had a significant reduction in lung tissues of Qingfei group; and Qingfei oral liquid had better regulatory effect on SOD, MDA and GSH than pirfenidone. The results of component-target-disease network and pathway enrichment analysis showed that the related molecular pathways were concentrated in inflammation, extracellular matrix and cytokines. Qingfei oral liquid has a good therapeutic effect on idiopathic pulmonary fibrosis in rats via regulation of inflammation, extracellular matrix and cytokines.

清瘟口服液对特发性肺纤维化大鼠的疗效及相关网络药理学研究
研究青飞口服液对特发性肺纤维化的治疗作用及机制。将72只雄性SD大鼠分为对照组、模型组、吡罗非酮组和清飞组,每组18只。后三组大鼠气管内注射博莱霉素诱导特发性肺纤维化,吡罗非酮组口服吡罗非酮b.i.d 21 d,清瘟组口服清瘟口服液3.6 mL/kg q.d,取肺组织进行HE染色、Masson染色和转化生长因子(TGF)-β免疫组化染色。组织匀浆中检测超氧化物歧化酶(SOD)、丙二醛(MDA)和谷胱甘肽(GSH)。利用 BATMAN-TCM 数据库,采用网络药理学方法检索了清妃口服液的化学成分及其相应的靶点,并构建了成分-靶点-疾病网络图。最后进行通路富集分析,探讨清瘟口服液抗特发性纤维化的分子机制。组织病理学结果显示,青飞口服液对肺纤维化的缓解作用与阳性药物吡非尼酮相似;青飞组肺组织中TGF-β分泌显著减少;青飞口服液对SOD、MDA和GSH的调节作用优于吡非尼酮。成分-靶标-疾病网络和通路富集分析结果表明,相关分子通路集中在炎症、细胞外基质和细胞因子。青飞口服液通过调节炎症、细胞外基质和细胞因子对大鼠特发性肺纤维化有较好的治疗效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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