Antibacterial Potential and Phytochemical Analysis of Barleria lupulina Lindl. (Aerial Parts) Extracts Against Respiratory Tract Pathogens

Pharmaceutical and Clinical Research Pub Date : 2017-07-25 DOI:10.25258/IJPCR.V9I7.8787

Ajeet Singh, Navneet

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引用次数: 1

Abstract

The antibacterial and phytochemical investigation of Barleria lupulina Lindl. aerial parts extracts were examined against common respiratory tract pathogens i.e., Streptococcus pneumoniae (MTCC 655), Staphylococcus aureus (MTCC 1144), Pseudomonas aeruginosa (MTCC 2474), Streptococcus pyogens (MTCC 442), Haemophillus influenzae (MTCC 3826). The plant material was extracted with solvents i.e., petroleum ether (PET), acetone (ACE), methanol (MeOH) and aqueous (H2O) with increasing polarity by Soxhlet apparatus and removed the solvent using vacuum evaporator at 30˚C. Antibacterial activity and minimum inhibitory concentration (MICs) were examined by Agar well diffusion two fold serial dilution method respectively. The maximum inhibition zone was found against S. aureus (17.34±0.78 mm) of methanol extract and minimum against S. pyogens (9.44±0.32 mm). MICs were observed for MeOH extract between 3.12 to 12.5 mg/mL against S. pneumoniae and S. pyogens respectively. Phytochemical examination of plant extracts showed the occurrence of alkaloids, saponins, steroids, flavonoids, glycosides, tannins, resins and phenolic compounds. The antimicrobial activity of the crude extracts of plant represents a significant outcome for the treatment of respiratory tract diseases.

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狼疮芽孢杆菌抑菌潜能及植物化学分析。(空中部分)抗呼吸道病原体提取物

狼疮芽孢杆菌的抑菌及植物化学研究。检测气参提取物对常见呼吸道病原菌肺炎链球菌(MTCC 655)、金黄色葡萄球菌(MTCC 1144)、铜绿假单胞菌(MTCC 2474)、致病菌链球菌(MTCC 442)、流感嗜血杆菌(MTCC 3826)的抑制作用。以石油醚(PET)、丙酮(ACE)、甲醇(MeOH)和极性递增的水(H2O)为溶剂，用索氏装置提取植物材料，在30℃的温度下用真空蒸发器除去溶剂。采用琼脂孔扩散两倍连续稀释法分别测定其抑菌活性和最低抑菌浓度(mic)。甲醇提取物对金黄色葡萄球菌的抑制区最大(17.34±0.78 mm)，对病原菌的抑制区最小(9.44±0.32 mm)。MeOH提取物对肺炎链球菌和化脓性链球菌的mic值分别为3.12 ~ 12.5 mg/mL。植物化学检测表明，植物提取物中含有生物碱、皂苷、甾体、黄酮类、苷类、单宁类、树脂类和酚类化合物。植物粗提取物的抗菌活性是呼吸道疾病治疗的重要成果。

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Pharmaceutical and Clinical Research

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