Nephroprotector activity of hydro alcoholic extract of Tinospora cordifolia roots on cisplatin induced nephrotoxicity in rats

Abstract

Objective

To investigate the protective role of the Tinospora cordifolia root extract against cisplatin induced nephrotoxicity in albino rats.

Methods

In the present study, the ethanol extract of roots of T. cordifolia (200 mg & 400 mg/kg bd.wt) was examined for its protective effect against cisplatin induced renal injury in rats. Thirty healthy male and female albino rats (150–200 g weight) were chosen and divided into five groups. Vehicle, cisplatin and ethanolic extract were given according to the experimental design. After 8 days of treatment, urinary functional parameters were analysed.

Results

Histopathological characters were examined after scarifying and dissecting the rats. Cisplatin (6 mg/kg) alone significantly elevated serum markers level, increased urinary protein excretion, reduced urine to serum creatinine ratio and creatinine clearance. In curative regimen, the extract significantly reduced the elevated serum creatinine and urea levels. Renal antioxidant defense systems, such as superoxide dismutase, catalase, glutathione peroxides activities and reduced glutathione level, depleted by cisplatin therapy were restored to normal by treatment with the extract.

Conclusion

The present investigation suggests that the hydro alcoholic extract of roots of T. cordifolia has protective effect against cisplatin induced nephrotoxicity.