Dietary Inclusion of Scent Leaf Meal (Ocimum Gratissimum) Affects Immune Genes Expression in Chicken Spleen at 28 and 56 Days

IF 0.5 4区农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Large Animal Review Pub Date : 2021-10-11 DOI:10.18488/journal.ar.2021.81.10.19

U. G. Sorhue, E. R. Onainor, A. M. Moemeka, Irikefe-Ekeke E Peterson

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引用次数: 2

Abstract

This study was conducted to examine the effects of scent leaf meal (Ocimum gratissimum) on expression of inflammatory cytokines in the spleen of two chicken strains. A total of 150birds (75 of each strain) were randomly allotted into five dietary treatments at fifteen birds per treatment. Birds were fed diet containing varying levels of Ocimum gratissimum leaf meal. Treatment one (T1) had 0% OG, while treatment two (T2), treatment three (T3), treatment four (T4) and treatment five (T5) had 0.5% OG, 1.00% OG, 1.5% OG and 2% OG respectively. Feed and water was provided adlibitum throughout the feeding trial. Three birds were slaughtered from each treatment at day 28 and day 56, spleen samples were collected and stored using RNALater in a -20oc freezer prior to RNA extraction. Real-time qPCR was performed in 40cycles using the PowerUp SYBR Green reagent and analyzed with the 2-∆∆Ct method. Gene expression data were subjected to two-way analysis of variance. Strain effect was significantly different (P<0.05) at both time points. All the genes studied significantly differed (P<0.05) in their expression patterns at 28 and 56days of age. Increased inclusion rate of the test ingredients significantly (P<0.05) reduced IL1β and NF-KB1, while increasing IL10 and NF-KB2. Ocimim gratissimum leaf meal shows promise in the regulation of inflammation in chickens and can be used to efficiently replace antibiotics in broiler production.

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饲粮添加香叶粕对28日龄和56日龄鸡脾脏免疫基因表达的影响

本试验研究了香叶粉对两株鸡脾脏炎性细胞因子表达的影响。150只鸡(每株75只)随机分为5个饲粮处理，每个处理15只。饲粮中添加了不同水平的茴香叶粉。处理1 (T1) OG为0%，处理2 (T2)、处理3 (T3)、处理4 (T4)和处理5 (T5) OG分别为0.5%、1.00%、1.5%和2%。在整个饲养试验期间，饲料和水是不平衡的。在第28天和第56天，每个处理宰杀3只鸡，收集脾脏样本，使用RNALater在-20℃冰箱中保存，然后提取RNA。采用PowerUp SYBR Green试剂进行Real-time qPCR，共40个循环，采用2-∆∆Ct法进行分析。基因表达数据进行双向方差分析。两个时间点应变效应差异有统计学意义(P<0.05)。各基因在28日龄和56日龄的表达量差异均显著(P<0.05)。增加试验成分的包合率显著(P<0.05)降低了il - 1β和NF-KB1，提高了il - 10和NF-KB2。枸杞叶粕在调节鸡机体炎症方面具有良好的应用前景，可在肉鸡生产中有效替代抗生素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Large Animal Review AGRICULTURE, DAIRY & ANIMAL SCIENCE-

CiteScore

0.90

自引率

16.70%

发文量

审稿时长

>12 weeks

期刊介绍： Large Animal Review is a bimonthly magazine published by SIVAR (Italian Society of Farm Animals Veterinary Practitioners) for scientific updating of veterinarians who deal with animals in livestock production and the supply chain control in the production of food industry. The topics of main interest for the journal are those of internal medicine, surgery, obstetrics, animal nutrition, zootechnics, infectious and parasitic diseases, food safety and security, animal welfare, prevention and management.