Evaluation effects of Quercetin on streptozotocin-treated RINm5F pancreatic β-cells in vitro

Maryam Mazraesefidi, Maryam Mohammad Sadeghipour, H. Khorramdelazad, Mahdi Mahmoodi, A. Khoshdel, M. Fahmidehkar, Reza Hosseiniara, M. Hajizadeh
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引用次数: 1

Abstract

Quercetin is a naturally occurring phenolic compound abundantly present in plants as a secondary metabolite. The purpose of this study was to investigate the effect of quercetin on improving RINm5F β-insulinemia cell viability, glucose-stimulated insulin secretion (GSIS), and cell insulin content in the presence or absence of streptozotocin (STZ). This experimental study was conducted on RINm5F β-insulinemia cell line. The cell viability was evaluated by MTT assay. The necrosis was confirmed by flowcytometry and insulin ELISA kit was used to measure the GSIS level and cell insulin content. It should be noted that for testing of cells by 50μM of quercetin, simultaneous treatment and pre-treatment of quercetin were performed in the presence of STZ (20mM). The quercetin was able to improve the viability of RINm5F cells in the presence of STZ and to increase the GSIS level and cell insulin content under STZ and glucotoxic conditions The quercetin seems to have beneficial effects on β-cells, especially the synthesis and secretion of insulin. In addition to the therapeutic effect, given the low toxicity of this flavonoid and the results of this study, the quercetin as a preventive agent may play an important role in maintaining the health of β-cells in people at risk of diabetes.
槲皮素对链脲佐菌素处理的RINm5F胰腺β细胞的体外影响
槲皮素是一种天然存在的酚类化合物,作为次生代谢物大量存在于植物中。本研究的目的是探讨槲皮素在存在或不存在链脲佐菌素(STZ)的情况下对改善RINm5F β-胰岛素血症细胞活力、葡萄糖刺激胰岛素分泌(GSIS)和细胞胰岛素含量的影响。本实验研究在RINm5F β-胰岛素血症细胞系上进行。MTT法测定细胞活力。流式细胞术证实坏死,胰岛素ELISA试剂盒检测GSIS水平及细胞胰岛素含量。需要说明的是,在50μM槲皮素的细胞检测中,槲皮素的预处理和处理都是在STZ (20mM)存在的情况下进行的。槲皮素能够在STZ存在的情况下提高RINm5F细胞的活力,增加STZ和糖毒性条件下的GSIS水平和细胞胰岛素含量。槲皮素似乎对β细胞,特别是胰岛素的合成和分泌有有益的作用。除了治疗效果外,鉴于这种类黄酮的低毒性和本研究的结果,槲皮素作为一种预防剂可能在维持糖尿病风险人群β-细胞的健康方面发挥重要作用。
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来源期刊
Current Chemical Biology
Current Chemical Biology Medicine-Biochemistry (medical)
CiteScore
1.40
自引率
0.00%
发文量
16
期刊介绍: Current Chemical Biology aims to publish full-length and mini reviews on exciting new developments at the chemistry-biology interface, covering topics relating to Chemical Synthesis, Science at Chemistry-Biology Interface and Chemical Mechanisms of Biological Systems. Current Chemical Biology covers the following areas: Chemical Synthesis (Syntheses of biologically important macromolecules including proteins, polypeptides, oligonucleotides, oligosaccharides etc.; Asymmetric synthesis; Combinatorial synthesis; Diversity-oriented synthesis; Template-directed synthesis; Biomimetic synthesis; Solid phase biomolecular synthesis; Synthesis of small biomolecules: amino acids, peptides, lipids, carbohydrates and nucleosides; and Natural product synthesis).
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