Changes in Haematological Parameters Following Toxicity Study with 80% Methanol Extract of Moringa oleifera in Wistar Rats

Abstract

To determine the acute toxicity effects of Moringa oleifera leaf extract on haematological parameters, twenty albino Wistar rats weighing between 108-254 g were split into four groups of fives rat each. Different doses of the extract were administered to the 3 groups and one group is used as control. Moringa oleifera 80% methanol leaf extract was administered at 2000 mg/kg to group, 1000 mg/kg to group, and 500 mg/kg while one group was used as control. The result recorded 4 deaths (60% mortality) in groups that received 2000 mg/kg and 1 death (20% mortality) in groups that received 1000 mg/kg. Blood samples were collected from the survived rats for hematological analysis. The result showed variation in Park cell volume (PCV), hemoglobin concentration (Hb), Red blood cells (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC). There are significant differences at p > 0.05, p > 0.01 and p > 0.001 between groups that exposed to different doses Moringa oleifera extract and the control group. The result also shows slight variation in total and differential leucocyte count, but all the values obtained from both treated and control groups are within the normal range. There are no recorded values of eosinophil, basophil and band cells in both the control and the experimental groups. From this result, it can be concluded that high dose of this extract caused alteration in normal red cells indices with significant differences, but have mild or no effect on total and differential leucocyte count. Hence, low toxicity of this plant made it safe and good medicinal agent and or supplement. Histopatological screening as well as phytochemical study to identify the compounds that affect the blood chemistry is highly recommended.