Effect of casein hydrolysate and paclobutrazol on embryogenesis of date palm (Phoenix dactylifera L.) cv. Nersy by in vitro culture

M. Ibrahim, H. A. Al-Taha, Zyad. T. S. AL-Ali
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引用次数: 1

Abstract

Results showed that fresh and dry weight of primary and embryogenic callus increased significant with increasing of casein or paclobutrazol after 16 weeks from culture. The treatments of 1000 mg.L -1 casein hydrolysate and 0.5 mg.L -1 paclobutrazol showed fewer periods to embryo formation reached 21.00 and 17.00 days, respectively. However, the control observed the longest period to somatic embryo formation which reached 61.00 days. The treatments of 1000 mg.L -1 casein hydrolysate and 1.0 mg.L -1 paclobutrazol showed the highest values 6.50 and 1.60 cm length, 0.87 and 0.80 cm diameter, 0.336 and 0.361 gm fresh weight and, 0.022 and 0.033 gm dry weight of embryo’s characteristics, respectively. In addition, two treatments of paclobutrazol (0.5 and 0.7 mg.L -1 ) showed high average number of embryos (16.33 and 16.67 embryos, respectively). In contrast, the recorded data from the control treatment gave demonstrated fewer values (4.33 embryos, 0.80 cm length, 0.13 cm diameter, 0.035 gm fresh weight and 0.005 gm dry weight) of characteristics of embryos. When the culture medium contained casein hydrolysate at 1000 mg.L -1 observed the highest percentage of germination (76%) and less period of embryo germination (15.30) days in comparison with other treatments. Moreover, the culture medium with low concentration of paclobutrazol (0.3 mg.L 1 ), showed the highest percentage of germination (75.67%) in comparison with other treatments of paclobutrazol. However, the same concentration of paclobutrazol obtained the lowest period of embryo germination (14.0 days) in comparison with the other treatments. The results also showed that the callus when cultured on MS medium supplemented with 800 or 1000 mg.L -1 casein hydrolysate demonstrated indirect adventitious shoots.
酪蛋白水解物和多效唑对枣椰树胚胎发生的影响。通过体外培养
结果表明:培养16周后,随着酪蛋白和多效唑含量的增加,初生和胚性愈伤组织的鲜重和干重显著增加;1000mg的处理。L -1酪蛋白水解物和0.5 mg。L -1多效唑的胚胎形成周期较短,分别为21.00和17.00 d。对照的体胚形成时间最长,达61.00 d。1000mg的处理。L -1酪蛋白水解物和1.0 mg。L -1多效唑对胚长6.50和1.60 cm、直径0.87和0.80 cm、鲜重0.336和0.361 gm、干重0.022和0.033 gm的影响最大。此外,多效唑(0.5和0.7 mg)两种处理。L -1)平均胚胎数较高,分别为16.33个和16.67个。相比之下,对照处理的记录数据显示胚胎特征值较少(4.33个胚胎,0.80 cm长,0.13 cm直径,0.035 gm鲜重和0.005 gm干重)。当培养基中含有1000毫克酪蛋白水解物时。与其他处理相比,L -1处理的发芽率最高(76%),发芽期最短(15.30)d。此外,低浓度多效唑(0.3 mg)的培养基。L 1),发芽率最高,为75.67%。而相同浓度多效唑处理的胚萌发期最低,为14.0 d。结果还表明,在添加800或1000 mg的MS培养基上培养愈伤组织。L -1酪蛋白水解产物表现为间接不定芽。
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