Exploitation of PGPR Endophytic Burkholderia Isolates to Enhance Organic Agriculture

Abstract

Although many bacterial species have been isolated from the rhizosphere of various crop plants, the recent discovery is Burkholderia sp., an endophytic bacterium. In this study, the Burkholderia isolates viz., RB1 (Rice Burkholderia 1), MB2 (Maize Burkholderia 2), SB3 (Sugarcane Burkholderia 3) and BB4 (Black gram Burkholderia 4) were enumerated from the root, stem and leaf samples of four different crops viz., rice, maize, sugarcane and black gram using N-free BAz (Burkholderia Azelaic acid) medium, in which black gram roots were observed higher population. Further, growth promoting activities of the Burkholderia isolates were examined, the maximum production of IAA and GA was noticed on the BB4 as compared to other isolates and the cytokinin production was recorded more in isolates SB3 followed by BB4. Among the four isolates, maximum amount of salicylate type was noticed in RB1 and catechol type was recorded higher in BB4 which showed that these isolates were capable to produce Siderophore. The ACC deaminase activity of the isolates were exhibited, the BB4 was recorded more followed by SB3. Therefore, the endophytic Burkholderia isolates also the important contributor to the crop growth through secretion of growth promoting substances, production of siderophore and ACC deaminase activities may improve the Agriculture production.