EXPERIMENTAL STUDIES ON THE DETECTION AND QUANTIFICATION OF VOLATILE FLAMMABLE AND TOXIC SUBSTANCES IN THE AIR AND BIOLOGICAL FLUIDS OF THE ORGANISM

Abstract

The methods of isolation, purification and qualitative and quantitative determination of phenol in air and biological fluids of an organism are offered. Isolation was carried out by distillation with water vapor, purification by extraction with chloroform, and identification by qualitative reactions, by chromatography in a thin layer of sorbent and by liquid chromatography. The quantitative determination of phenol was carried out by liquid chromatography in a reverse phase (chromatograph "Tsvet-304" with an ultraviolet detector). Calculation of the quantitative content of phenol was carried out by the method of absolute calibration. As a result of the research, it was found that by using the molecular liquid chromatography method, it is possible to determine 17-20% of phenol isolated from urine and 21-24% isolated from the air. The retention time of phenol 1h.14s, the results of the analysis are well reproducible. The worked out techniques have high resolution, high sensitivity and speed of analysis.