Antifungal activity screening and HPLC analysis of crude extract from Tectona grandis, Shilajit, Valeriana wallachi.

Electronic journal of environmental, agricultural and food chemistry Pub Date : 2007-12-31 DOI:10.5580/2390

Shalini, R. Srivastava

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引用次数: 51

Abstract

The antifungal activity of methanolic crude extract of Tectona grandis, Shilajit, Valeriana wallachi was investigated against Alternaria cajani, Curvularia lunata, Fusarium sp., Bipolaris sp. and Helminthosporium sp. at different concentrations (1000, 2000, 3000, 4000 and 5000 �og/ml). Better antifungal activity was observed with the extracts of Valeriana wallachi, that showed excellent inhibitory activity against Helminthosporium sp. (96.15%) followed by Shilajit extract against Alternaria cajani (95.12 %) and Helminthosporium sp. (95.00 %) at concentration of 5000 �og/ml. Among different fungi tested Bipolaris sp. and Fusarium were found to be more sensitive to crude extract when compared to others. The increase in the production of phenolics in the extract can be correlated with the induction of resistance in treated plants against phytopathogenic fungi. HPLC analysis of the crude extract of medicinal plants showed four different Phenolic acids (Tannic acid, Gallic acid, Ferulic acid and Caffeic acid). The results of the study provide scientific basis for the use of the plant extract in the future development as antioxidant, antibacterial, antifungal and anti-inflammatory agent.

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龙葵、石竹、缬草粗提物抑菌活性筛选及HPLC分析。

研究了不同浓度(1000、2000、3000、4000和5000 μ g/ml)的大构造草(Tectona grandis)、石竹(Shilajit)、缬草(Valeriana wallachi)甲醇粗提物对稻蚀菌(Alternaria cajani)、月曲菌(Curvularia lunata)、镰刀菌(Fusarium sp.)、双极菌(Bipolaris sp.)和Helminthosporium sp.)的抑菌活性。缬草提取物对线虫的抑菌活性较好，在5000 μ g/ml浓度下，对线虫的抑菌活性最高(96.15%)，其次是夏拉吉特提取物(95.12%)和线虫的抑菌活性最高(95.00%)。在不同的真菌测试中，发现双星菌和镰刀菌对粗提取物的敏感性较强。提取物中酚类物质产量的增加可能与处理过的植物对植物病原真菌的抗性诱导有关。药用植物粗提物的高效液相色谱分析显示四种不同的酚酸(单宁酸、没食子酸、阿魏酸和咖啡酸)。研究结果为今后开发利用该植物提取物作为抗氧化、抗菌、抗真菌和抗炎剂提供了科学依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Electronic journal of environmental, agricultural and food chemistry

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