Studies on Antimicrobial Activity and Characteristics of Bacteriocins Produced by Lactobacillus strains Isolated from Milk of Domestic Animals

Abstract

Lactobacilli strains were isolated from the milk of domestic animals for potential probiotic. A total of 120 milk samples (40 each from buffalo, cow and goat) were analyzed and 110 Lactic Acid Bacteria (LAB) were identified, out of these 43 were identified as probiotics, (L. acidophilus, L. bulgaricus, L. plantarum, L. lactis and L. rhamnosus). Out of these, 11 isolates were potential probiotics, which includes L. plantarum (C1, C4, C7, G7, G8 and B14) and L. rhamnosus (B13, C5, G4, G10 and G18). Their bacteriocins showed a broad inhibitory spectrum against the indicator organisms tested. The bacteriocins produced by L. plantarum (C4, G7) and L. rhamnosus (G18) showed prominent antimicrobial activity, resistance to heat at 121 C and tolerate acidic pH 3 but sensitive to pH 9 indication strong probiotic potential. These isolated LABS, exhibiting excellent probiotic characteristics, can be use in the protection and improvement of intestinal microbial flora and contribute health benefits to consumers.