Isolation and screening of different isolates of Aspergillus for amylases production

Abstract

The diversity of Aspergillus from soil of Kusmi Forest, an Indian subtropical Teak forest at Gorakhpur resulted in characterizing a total of 415 isolates which were then subjected to amylases production. The preliminary amylases production revealed a total of 14 promising isolates of Aspergillus comprising of ten different strains of A. flavus, two strains of A. tamarii, one strain each of A. niger and A. awamori which are characterized further. The optimum temperature of amylases produced from 14 isolates ranged from 30 to 60 C while thermal stability observed was in the range of 50 to 80 C for 30 minutes. The pH optima ranged from 5.0 to 8.6 and the Km values calculated ranged from 0.60 to 2.85 mg/ml.