Antifungal and Synergistic Effects of the Ethyl Acetate Extract of Tanacetum vulgare (L.) Against Candida albicans.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2019-08-27 DOI:10.12659/MSMBR.917394

Ariana Kameri, Ferit Koçani, Zeqir Hashani, Kemajl Kurteshi, Blerim Kamberi, Arsim Kurti, Arben Haziri

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Abstract

BACKGROUND With the continued demand for new, effective, and safe endodontic therapies, the aim of this study was assessment of efficiency of the ethyl acetate (EthOAc) extract of Tanacetum vulgare (L.) against Candida albicans. MATERIAL AND METHODS The antifungal effectiveness of the EthOAc extract of T. vulgare was determined using the agar disk diffusion method. The inhibition zones induced by the EthOAc extract were compared after 5 minutes, 60 minutes, and 24 hours to those induced by standard solutions (2% chlorhexidine, saturated calcium hydroxide, and 2% sodium hypochlorite). Statistical analysis of the results was performed using the Kruskal-Wallis test and one-way ANOVA. RESULTS The inhibition zone of chlorhexidine against C. albicans was 30.3-19.3 mm, but in combination with EthOAc extract (100 mg/mL) of T. vulgare, this inhibition was from 32.7-30 mm, indicating that this combination exerted a marked synergistic effect against C. albicans. The inhibition zone of sodium hypochlorite (69.7-65 mm) was higher than the inhibition zone of EthOAc extract and chlorhexidine. The combination of EthOAc extract with sodium hypochlorite resulted in a loss of antifungal activity. Furthermore, the activity of the EthOAc extract against C. albicans was decreased after mixing the extract with dentine at a concentration of 25 mg/50 µL (30.3-20.7 mm). The EthOAc extract did not show a genotoxic effect on lymphocyte cells. CONCLUSIONS The EthOAc extract of T. vulgare may be a useful tool to discover natural bioactive agents that have antifungal activity against C. albicans and could be used as endodontic therapies.

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醋酸乙酯提取物对白色念珠菌的抗真菌和协同作用

背景随着对新的、有效的和安全的牙髓疗法的不断需求，本研究的目的是评估虎耳草乙酸乙酯（EthOAc）提取物对白色念珠菌的功效。材料与方法采用琼脂盘扩散法测定丹参乙酸乙酯提取物的抗真菌效果。将 5 分钟、60 分钟和 24 小时后乙氧基羰基萃取物诱导的抑菌区与标准溶液（2% 洗必泰、饱和氢氧化钙和 2% 次氯酸钠）诱导的抑菌区进行比较。采用 Kruskal-Wallis 检验和单因素方差分析对结果进行统计分析。结果洗必泰对白僵菌的抑制区为 30.3-19.3 毫米，但与秃头椿的乙氧基钙提取物（100 毫克/毫升）联合使用时，抑制区为 32.7-30 毫米，表明这种联合使用对白僵菌有明显的协同作用。次氯酸钠的抑菌区（69.7-65 毫米）高于 EthOAc 提取物和洗必泰的抑菌区。将乙氧基羰基萃取物与次氯酸钠混合使用会导致抗真菌活性下降。此外，将浓度为 25 mg/50 µL 的乙酸乙酯提取物与牙本质混合后，乙酸乙酯提取物对白茨球菌的活性也有所下降（30.3-20.7 mm）。EthOAc提取物对淋巴细胞没有遗传毒性作用。结论 T. vulgare 的乙氧基羰基萃取物可能是一种有用的工具，可用于发现对白癣菌具有抗真菌活性的天然生物活性剂，并可用作牙髓治疗。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.