SCREENING OF BIOACTIVE COMPONENTS BY GC-MS ANALYSIS AND THE STUDY ON IN VITRO ANTICANCER EFFICACY OF MICROPORUS AFFINIS (BLUME & T. NEES) KUNT.

International Research Journal Of Pharmacy Pub Date : 2020-12-31 DOI:10.7897/2230-8407.1112104

S. Aneesh, J. E. Thoppil

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Abstract

Natural compounds with biological activity are normally present in plants, mushrooms and their natural sources. Applied mycology is one of the most stimulating and rapidly evolving areas of the biological sciences. Hence the present study focussed on exploring Microporus affinis (Blume & T. Nees) Kunt., the least explored and edible bracket fungus. Chemical characterization by GC-MS analysis resulted in the presence of 47 bioactive compounds. 9, 12- Octadecadienoic acid (Z,Z)- methyl ester, Ergosterol, Monolinolein, Thiacremonone, Stellasterol, n- Hexadecanoic acid, Ribitol, Maltol etc., were the leading compounds. Because of the presence of various bioactive compounds which have been already reported to possess antitumor, antioxidant and anticancer activities, M. affinis extract has been tested for in vitro anticancer efficacy on DLD1 cell lines (cultured in DMEM medium) using MTT assay. It resulted in the decrease of percentage of viability as the increase in concentration of the extract. Apoptosis was determined by using Acridine orange and Ethidium bromide staining. Thus, the taxa, M. affinis can be recommended for further anticancer assays for validation.

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气相色谱-质谱联用法筛选affinis microporus (blume & t. nees) kunt的活性成分及体外抗癌作用研究。

具有生物活性的天然化合物通常存在于植物、蘑菇及其天然来源中。应用真菌学是生物科学中最令人兴奋和发展最快的领域之一。因此，本研究的重点是探索Microporus affinis (Blume & T. Nees) Kunt。是探索最少的可食用支架菌。经GC-MS分析，共鉴定出47种生物活性化合物。9,12 -十八二烯酸(Z,Z)-甲酯、麦角甾醇、单油酸、硫胺酮、星甾醇、正十六烷酸、利比醇、麦芽醇等为先导化合物。由于存在多种生物活性化合物，已报道具有抗肿瘤，抗氧化和抗癌活性，因此使用MTT法对DLD1细胞株(在DMEM培养基中培养)进行了体外抗癌效果测试。结果表明，随着提取物浓度的增加，活性百分率降低。用吖啶橙和溴化乙啶染色法检测细胞凋亡。因此，类群M. affinis可以推荐用于进一步的抗癌试验以进行验证。

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International Research Journal Of Pharmacy

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