Comparative assessment of ISSR, DAMD and RAPD markers for evaluation of genetic diversity of gerbera (Gerbera jamesonii Bolus ex Hooker f.) cultivars

Acta agriculturae Slovenica Pub Date : 2023-04-28 DOI:10.14720/aas.2023.119.1.2425

A. Saidi, M. Hajkazemian

引用次数: 0

Abstract

Genetic diversity is the best way to improve available genetic resources for breeding programs in gerbera. In present study, genetic diversity of 22 gerbera cultivars was investigated using inter-simple sequence repeat (ISSR), directly amplified minisatellite DNA (DAMD), and Random Amplified Polymorphic DNA (RAPD) markers. Average polymorphism information content (PIC) for ISSR, DAMD and RAPD markers was 0.40, 0.41 and 0.40, respectively. Cluster analysis for ISSR, DAMD and RAPD divided the cultivars into three distinct clusters. The comparative analysis of the three markers (ISSR, DAMD and RAPD) showed that DAMD had superiority over RAPD and ISSR in characterization of genetic diversity in Gerbera. To our knowledge, this is the first report of a comparison of performance among DAMD, ISSR and RAPD techniques on a set of gerbera genotypes. Overall, our results showed that DAMD markers well represented different genotypes of gerbera diversity.

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利用ISSR、DAMD和RAPD标记评价非洲菊(gerbera jamesonii Bolus ex Hooker f.)品种遗传多样性

遗传多样性是改善非洲菊育种计划可用遗传资源的最佳途径。采用简单序列重复序列(ISSR)、直接扩增微卫星DNA (DAMD)和随机扩增多态性DNA (RAPD)标记对22个非洲菊品种的遗传多样性进行了研究。ISSR、DAMD和RAPD标记的平均多态性信息含量(PIC)分别为0.40、0.41和0.40。ISSR、DAMD和RAPD聚类分析将品种划分为3个不同的聚类。对3个标记(ISSR、DAMD和RAPD)的比较分析表明，DAMD在非洲菊遗传多样性鉴定方面优于RAPD和ISSR。据我们所知，这是第一份比较DAMD、ISSR和RAPD技术在一组非洲菊基因型上表现的报告。总体而言，我们的结果表明，DAMD标记很好地代表了非洲菊不同基因型的多样性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta agriculturae Slovenica Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

0.90

自引率

0.00%

发文量

期刊介绍： Acta agriculturae Slovenica is a scientific journal published quarterly by the Agronomy, and the Zootechnical Departments of the Biotechnical Faculty, University of Ljubljana, Slovenia. Source of high quality research from the field of agronomy, zootechnics, applied biology and related fields from Slovenia and other countries.