Optimization of a Method for Obtaining and Determination of Carotenoids in Tambukan Mud

D. Kompantsev, A. Chakhirova, T. Gutnova
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Abstract

— Therapeutic sulphide silt mud from Tambukan lake has been formed for a long period of time. Balneological value of silt mud from that specific lake is high because of high level of ferrous sulphides and water-soluble salts in it’s content, including recognized as therapeutically active bromine and boric acid; high concentration of lipids and carotenoids, which have anti-inflammatory, anti-bacteria, immune stimulating and bio stimulating effects. Goal: development of the method for stimulation of the peloid extract obtaining process from the Tambukan mud by an ultrasound-assisted extraction with further determination of biologically active substance using a spectrophotometric method. The research is based on formerly known and patented methods, offered by Kh.G.Karagulov. The method is reasonable, allows to extract carotenoid in full. The disadvantage of it is the energy-demanding and the usage of an organic solvent. As the object of the research a deep seated mud sample from the Tambukan lake was taken. After an ultrasound-assisted extraction there was obtained an oily extract from the mud sample. Quantitative content estimation was held out using a spectrophotometric method in visible region of absorption. The ability to optimize the process of peloid extraction from the Tambukan mud by the ultrasound-assisted extraction has been proven. Offered method for extraction and determination of carotenoid amount in Tambukan mud is less resource- and energy-demanding. Alongside with that it provides best possible carotenoid amount in the outcome. Effective and less expensive method to obtain peloid from Tambukan mud has been developed. The research results point out that the level of carotenoids in Tambukan silt mud has been reduced resulting in serious reduction of therapeutic effect. in
坦布坎泥中类胡萝卜素提取及含量测定方法的优化
-坦布坎湖的治疗性硫化物淤泥已经形成了很长一段时间。该湖淤泥中硫化铁和水溶性盐含量高,包括具有治疗活性的溴和硼酸,因此淤泥的生物学价值很高;高浓度脂类及类胡萝卜素,具有抗炎、抗菌、免疫刺激及生物刺激作用。目的:建立超声辅助提取法对坦布坎泥类提取物提取工艺的刺激,并进一步采用分光光度法测定生物活性物质。这项研究是基于karagulov提供的以前已知和专利的方法。方法合理,可充分提取类胡萝卜素。它的缺点是能源需求和使用有机溶剂。研究对象为坦布坎湖深层泥样。超声辅助萃取后,从泥浆样品中获得了一种油性萃取物。用分光光度法在可见吸收区进行了定量含量估计。研究结果表明,超声辅助提取法可以优化坦布坎泥质体的提取工艺。提出的提取和测定坦布坎泥中类胡萝卜素含量的方法对资源和能量的要求较低。与此同时,它提供了最好的类胡萝卜素量的结果。开发了一种从坦布坎泥浆中提取似球粒的有效而经济的方法。研究结果表明,坦布坎淤泥中类胡萝卜素含量降低,导致治疗效果严重下降。在
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