Biodegradation of Endosulfan as an Organochlorine Pesticide with Pseudomonas plecoglocissida Transfected by LinA Gene

Abstract

Background: Organochlorinepesticides(OC)areappliedtosoilandagriculturalfarmsandcancontaminatesewerageandditches, which in turn may be transported to rivers, and consequently distributed in the environment and as a pollutant that endangers human health. Objectives: This study aimed at constructing a genetically engineered Pseudomonas plecoglossicida strain enabled to degrade the OC pesticide, endosulfan. Methods: Transfected Pseudomonas plecoglossicida strain was initially made in the following steps, first polymerase chain reaction (PCR) product of gene LinA from Sphingomonas paucimobilis UT26 was transferred to the pUC57 plasmid, and the plasmid was then transfected to P. plecoglossicida . Results: In endosulfan supplemented carbon-deficient minimal medium, the isolate showed its ability to hydrolyze endosulfan to endosulfansulphate. Theisolatewasobservedtogrowonendosulfanasthecarbonsource. Thus,thestrainwascapableofdegrading the OC pesticides and utilizing products of their degradation as nutrients. Conclusions: Ability of transfected Pseudomonas plecoglossicida strain in biodegradation of chemicals makes it an excellent candidate for bioremediation of contaminated agricultural and industrial sites.