Chemical Constituents of Indonesian Micromelum minutum Leaves and Their Cytotoxicity Against MCF-7 and 4T1 Breast Cancer Cells

IF 0.5 Q4 MULTIDISCIPLINARY SCIENCES

Journal of Mathematical and Fundamental Sciences Pub Date : 2021-06-07 DOI:10.5614/J.FUND.MATH.SCI.2021.53.1.7

R. A. Susidarti, E. Meiyanto, Muthi’ Ikawati, Normaidah Normaidah, Nurramadhani A Sida

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Abstract

Micromelum minutum is used widely in traditional folk medicine. Although this species has been investigated extensively and several bioactive compounds have been isolated, little work has been done on Indonesian M. minutum. This research aimed to study the chemical constituents and biological activities of M. minutum cultivated in Bantimurung Bulusaraung National Park, South Sulawesi, Indonesia. The isolated compounds were assessed for their cytotoxicity towards MCF-7 and 4T1 cell lines by MTT method. The dried ground leaves of M. minutum were sequentially macerated with n-hexane, ethyl acetate, and methanol. The n-hexane and ethyl acetate extracts contained a flavonoid 5,7-dihydroxy-3,4',8-trimethoxyflavone (1) which inhibited MCF-7 and 4T1 cell viability by 50% at concentrations of 369±8 and 227±5 µM, respectively. Further separation of the ethyl acetate extract by column chromatography yielded acetyldihydromicromelin A (2) and a mixture of dihydromicromelin A (3) and dihydromicromelin B (4), which were not active toward MCF-7 and 4T1 cells.

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印尼微香叶化学成分及其对MCF-7和4T1乳腺癌细胞的细胞毒性研究

小茴香在传统民间医学中应用广泛。虽然人们对该物种进行了广泛的研究，并分离出了几种生物活性化合物，但对印度尼西亚M. minutum的研究却很少。本研究旨在研究印度尼西亚南苏拉威西邦提姆隆布鲁萨朗国家公园种植的石竹的化学成分和生物活性。采用MTT法对分离得到的化合物对MCF-7和4T1细胞株进行细胞毒性评价。分别用正己烷、乙酸乙酯和甲醇浸渍干磨叶。正己烷和乙酸乙酯提取物中含有类黄酮5,7-二羟基-3,4'，8-三甲氧基黄酮(1)，在浓度为369±8µM和227±5µM时，MCF-7和4T1细胞活力分别下降50%。乙酸乙酯萃取物进一步用柱层析分离得到乙酰二氢微melin A(2)和二氢微melin A(3)和二氢微melin B(4)的混合物，它们对MCF-7和4T1细胞没有活性。

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来源期刊

Journal of Mathematical and Fundamental Sciences MULTIDISCIPLINARY SCIENCES-

CiteScore

1.30

自引率

0.00%

发文量

审稿时长

24 weeks

期刊介绍： Journal of Mathematical and Fundamental Sciences welcomes full research articles in the area of Mathematics and Natural Sciences from the following subject areas: Astronomy, Chemistry, Earth Sciences (Geodesy, Geology, Geophysics, Oceanography, Meteorology), Life Sciences (Agriculture, Biochemistry, Biology, Health Sciences, Medical Sciences, Pharmacy), Mathematics, Physics, and Statistics. New submissions of mathematics articles starting in January 2020 are required to focus on applied mathematics with real relevance to the field of natural sciences. Authors are invited to submit articles that have not been published previously and are not under consideration elsewhere.