{"title":"Molecular docking, isolation and biological evaluation of Rhizophora mucronata flavonoids as anti-nociceptive agents","authors":"Selvaraj Gurudeeban , Satyavani Kaliamurthi , Haja Sherief Sheik , Ramanathan Thiruganasambandam","doi":"10.1016/j.bionut.2014.08.002","DOIUrl":null,"url":null,"abstract":"<div><p>The anti-nociceptive effect of <em>Rhizophora mucronata</em><span> was evaluated on chemically and thermally induced nociception in mice. Albino mice received a dose of 10, 15, 20, or 25</span> <!-->mg/kg of alkaline chloroform fraction (Alk-CF) of <em>R</em>. <em>mucronata</em><span> by oral administration. Compared with controls, Alk-CF decreased the writhing numbers (</span><em>P</em> <!--><<!--> <span><span>0.01) in a dose-dependent manner. Further, we determined that Alk-CF contained, a potent compared to control, also potent anti-nociceptive agent that acted via opioid receptors and using </span>HPLC<span>, identified this compound as luteolin<span>. Docking simulation demonstrated that luteolin interacted strongly with cyclooxygenase, forming a number of specific hydrogen bonds. This study identified peripheral and central anti-nociceptive activities of </span></span></span><em>R. mucronata</em> that involve opioid receptor, and in which the active compound is luteolin as a source of new anti-nociceptive agent.</p></div>","PeriodicalId":100182,"journal":{"name":"Biomedicine & Preventive Nutrition","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2014-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bionut.2014.08.002","citationCount":"9","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedicine & Preventive Nutrition","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2210523914000798","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 9
Abstract
The anti-nociceptive effect of Rhizophora mucronata was evaluated on chemically and thermally induced nociception in mice. Albino mice received a dose of 10, 15, 20, or 25 mg/kg of alkaline chloroform fraction (Alk-CF) of R. mucronata by oral administration. Compared with controls, Alk-CF decreased the writhing numbers (P < 0.01) in a dose-dependent manner. Further, we determined that Alk-CF contained, a potent compared to control, also potent anti-nociceptive agent that acted via opioid receptors and using HPLC, identified this compound as luteolin. Docking simulation demonstrated that luteolin interacted strongly with cyclooxygenase, forming a number of specific hydrogen bonds. This study identified peripheral and central anti-nociceptive activities of R. mucronata that involve opioid receptor, and in which the active compound is luteolin as a source of new anti-nociceptive agent.