Antibacterial Activity of ZnO Nanoparticle Prepared by Pulsed Laser Ablation in Liquid for Biological Sensor

Abstract

The paper studies physical and antibacterial properties of ZnO nanoparticles obtained by pulsed laser ablation in water and ethanol. The energy used is 1000 mJ and 500 mJ for samples prepared in distilled water and ethanol and 1 Hz is installed while the temperature is 27°C and the number of pulses for both energies is 15 pulses. We have used Uv-visible spectroscopy, atomic force microscopy, the x-ray diffraction system and antibacterial activity of the nanoparticles was examined by its affection on gram- positive Staphylococcus aureus (S.aureus), gram-negative Pseudomonas and Candeda fungus. The dependence of nanoparticles’ physical and antibacterial properties on the conditions of the ablation was shown. Biochemical assays showed that the zinc recorded in distilled water and 1000 mJ and 500 mJ was fatal for all bacteria used in this study, whereas the zinc recorded in ethanol was not proven effective.