No Association Between His 447 His Polymorphism of PPARγ Gene and Osteoporosis in Iranian Postmenopausal Women

Abstract

Background: Osteoporosis is frequently observed in postmenopausal women. Evidence indicates that the role of genetic factors is significant in osteoporosis. This study aimed to inspect the potential association between His 447 His polymorphism of PPARγ gene and bone mineral density (BMD) and serum lipid profiles in postmenopausal women suffering from osteoporosis compared to the healthy controls. Methods: The study was conducted on 224 postmenopausal women including 107 osteoporosis patients (age: 61.03 ± 4.54 years) and 117 healthy controls (age: 55.73 ± 2.08 years). Blood samples were analyzed for polymorphism of PPARγ gene using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) methods. Multivariate analysis was used to investigate the relationship between the risk of osteoporosis and PPARγ gene polymorphisms. Results: Serum low density lipoprotein (LDL) and total cholesterol (TC) levels were significantly different between the osteoporosis group and the control group (P < 0.001). After adjustment for factors such as TC and LDL, logistic regression analysis showed that the risk of osteoporosis was not significantly higher in carriers of homozygous wild-type genotype than carriers of the rare alleles (P = 0.99, odds ratio = 0.997, 95% confidence interval = 0.52 - 1.91). Conclusions: This study suggests that His 447 His polymorphism of PPARγ gene does not play the role of an independent factor in BMD and osteoporosis in postmenopausal women. Keywords: Osteoporosis, Postmenopausal Women, Bone Mineral Density, Pparγ, His 447 His Gene Polymorphism