{"title":"Expression and significance of α2 subunit of 20s proteasome in anterior capsule of lens in patients with age-related cataract","authors":"Dandan Su, Jiusheng Zheng","doi":"10.3760/CMA.J.ISSN.1006-4443.2017.05.023","DOIUrl":null,"url":null,"abstract":"Objective \nTo identify the relationship between the degree of lens opacity and the hardness of nuclear and the protein expression level of 20s proteasome α2 subunit in age-related cataract patients. \n \n \nMethods \nPhacoemulsification or small incision cataract extraction was performed in 120 cases (60 cases of nuclear and 60 cases of cortical) and collected these anterior capsules. According to the degree of hardness and opacity of the lens nucleus, the nucleus was divided into group A: young nucleus, group B: old nuclear; cortical was divided into group A: mild opacity, group B: severe haze. Two types of cataract in group A, group B; and Western-blot was used to determine the 20s proteasome subunit alpha 2 expression levels in two groups of patients with ARC in the anterior lens capsule; experimental research work from July 2015 to March 2016. \n \n \nResults \nCompared with group A, the group B, 20s proteasome subunit α2 expression levels were significantly lower (nuclear (0.99±0.02) VS (0.58±0.08) P <0.01, cortical (1.26±0.15) VS (0.89±0.09), P <0.05, with the degree of lens opacity and nuclear hardness with the 20s proteasome α2 subunit content in age-related cataract patients was negatively correlated. \n \n \nConclusions \nThe protein expression levels of 20s protein α2 subunit in the anterior capsule of age-related cataract patients decrease with the degree of lens opacity and the hardness of the nucleus. \n \n \nKey words: \nAge-related cataract; 20s proteasome α2 subunit; Ubiquitin proteasome pathway; Anterior capsule","PeriodicalId":10236,"journal":{"name":"中国实用眼科杂志","volume":"51 1","pages":"533-537"},"PeriodicalIF":0.0000,"publicationDate":"2017-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中国实用眼科杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1006-4443.2017.05.023","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective
To identify the relationship between the degree of lens opacity and the hardness of nuclear and the protein expression level of 20s proteasome α2 subunit in age-related cataract patients.
Methods
Phacoemulsification or small incision cataract extraction was performed in 120 cases (60 cases of nuclear and 60 cases of cortical) and collected these anterior capsules. According to the degree of hardness and opacity of the lens nucleus, the nucleus was divided into group A: young nucleus, group B: old nuclear; cortical was divided into group A: mild opacity, group B: severe haze. Two types of cataract in group A, group B; and Western-blot was used to determine the 20s proteasome subunit alpha 2 expression levels in two groups of patients with ARC in the anterior lens capsule; experimental research work from July 2015 to March 2016.
Results
Compared with group A, the group B, 20s proteasome subunit α2 expression levels were significantly lower (nuclear (0.99±0.02) VS (0.58±0.08) P <0.01, cortical (1.26±0.15) VS (0.89±0.09), P <0.05, with the degree of lens opacity and nuclear hardness with the 20s proteasome α2 subunit content in age-related cataract patients was negatively correlated.
Conclusions
The protein expression levels of 20s protein α2 subunit in the anterior capsule of age-related cataract patients decrease with the degree of lens opacity and the hardness of the nucleus.
Key words:
Age-related cataract; 20s proteasome α2 subunit; Ubiquitin proteasome pathway; Anterior capsule
期刊介绍:
China Practical Ophthalmology was founded in May 1983. It is supervised by the National Health Commission of the People's Republic of China, sponsored by the Chinese Medical Association and China Medical University, and publicly distributed at home and abroad. It is a national-level excellent core academic journal of comprehensive ophthalmology and a series of journals of the Chinese Medical Association.
China Practical Ophthalmology aims to guide and improve the theoretical level and actual clinical diagnosis and treatment ability of frontline ophthalmologists in my country. It is characterized by close integration with clinical practice, and timely publishes academic articles and scientific research results with high practical value to clinicians, so that readers can understand and use them, improve the theoretical level and diagnosis and treatment ability of ophthalmologists, help and support their innovative development, and is deeply welcomed and loved by ophthalmologists and readers.