Expression and significance of α2 subunit of 20s proteasome in anterior capsule of lens in patients with age-related cataract

Dandan Su, Jiusheng Zheng
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Abstract

Objective To identify the relationship between the degree of lens opacity and the hardness of nuclear and the protein expression level of 20s proteasome α2 subunit in age-related cataract patients. Methods Phacoemulsification or small incision cataract extraction was performed in 120 cases (60 cases of nuclear and 60 cases of cortical) and collected these anterior capsules. According to the degree of hardness and opacity of the lens nucleus, the nucleus was divided into group A: young nucleus, group B: old nuclear; cortical was divided into group A: mild opacity, group B: severe haze. Two types of cataract in group A, group B; and Western-blot was used to determine the 20s proteasome subunit alpha 2 expression levels in two groups of patients with ARC in the anterior lens capsule; experimental research work from July 2015 to March 2016. Results Compared with group A, the group B, 20s proteasome subunit α2 expression levels were significantly lower (nuclear (0.99±0.02) VS (0.58±0.08) P <0.01, cortical (1.26±0.15) VS (0.89±0.09), P <0.05, with the degree of lens opacity and nuclear hardness with the 20s proteasome α2 subunit content in age-related cataract patients was negatively correlated. Conclusions The protein expression levels of 20s protein α2 subunit in the anterior capsule of age-related cataract patients decrease with the degree of lens opacity and the hardness of the nucleus. Key words: Age-related cataract; 20s proteasome α2 subunit; Ubiquitin proteasome pathway; Anterior capsule
年龄相关性白内障患者晶状体前囊20s蛋白酶体α2亚基的表达及意义
目的探讨老年性白内障患者晶状体混浊程度与晶状体核硬度及20s蛋白酶体α2亚基蛋白表达水平的关系。方法对120例(核性60例,皮质性60例)行超声乳化术或小切口白内障摘除术,收集前囊。根据晶状体核的硬度和不透明程度,将晶状体核分为A组:年轻核,B组:老核;皮层分为A组轻度浑浊,B组重度浑浊。A、B组白内障分两种;Western-blot检测两组前晶状体囊内20s蛋白酶体亚单位α 2的表达水平;2015年7月至2016年3月的实验研究工作。结果与A组、B组比较,20s蛋白酶体α2亚基表达水平显著降低(核(0.99±0.02)VS(0.58±0.08)P <0.01,皮质(1.26±0.15)VS(0.89±0.09)P <0.05,且晶状体不透明程度和核硬度与20s蛋白酶体α2亚基含量在年龄相关性白内障患者中呈负相关。结论年龄相关性白内障前囊20s蛋白α2亚基的表达水平随晶状体混浊程度和晶核硬度的增加而降低。关键词:老年性白内障;20s蛋白酶体α2亚基;泛素蛋白酶体途径;前囊
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来源期刊
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期刊介绍: China Practical Ophthalmology was founded in May 1983. It is supervised by the National Health Commission of the People's Republic of China, sponsored by the Chinese Medical Association and China Medical University, and publicly distributed at home and abroad. It is a national-level excellent core academic journal of comprehensive ophthalmology and a series of journals of the Chinese Medical Association. China Practical Ophthalmology aims to guide and improve the theoretical level and actual clinical diagnosis and treatment ability of frontline ophthalmologists in my country. It is characterized by close integration with clinical practice, and timely publishes academic articles and scientific research results with high practical value to clinicians, so that readers can understand and use them, improve the theoretical level and diagnosis and treatment ability of ophthalmologists, help and support their innovative development, and is deeply welcomed and loved by ophthalmologists and readers.
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