Isolation of lymphocytes from blood. A procedure combining nylon fibre filtration and differential centrifugation.

Abstract

Lymphocytes were isolated from human blood by a simple procedure combining nylon fibre filtration and centrifugation in colloidal silica-polyvinylpyrrolidone. In the preparation obtained 99.5 ± 0.6 per cent of the leukocytes were lymphocytes; it contained 0–202, mostly less than 20, platelets, and 0–1.5, mostly 0, RBC per 100 leukocytes. The lymphocyte recovery was 13.3 ± 3.3 per cent. In the trypan blue test 2.2 ± 3.0 per cent of the cells were stained.