Hepatorenal Effects of Silver Nanoparticles in In-Vivo Postnatal Model of Toxicity and in HepG2 Cell Line

Abstract

Silver nanoparticles (Ag-NPs) had many uses in medicine, household and industry. To better understand the postnatal toxicity of Ag-NPs in lactating female rats and its offspring’s, 18 female rats after delivery were divided into three groups and dams received orally the AGNPs at doses of 0, 50, 100 ppm daily for 21 days. After the end of treatment, all rats were euthanized and blood and tissues were separated for evaluation of biochemical and histopathology in dams and its pups. The Ag-NPs had no effect on the dam's weight while the reduction of rats’ pups weight was noticed after first week only after the treatment. Notably, Ag-NPs had toxic effects in rat’s pups, as well as its dam with evidence of elevation of liver enzymes, urea, creatinine and reduction of serum protein, albumin and globulin and considered the first report explained the toxicity in the rat’s pups. Moreover, rats' pups revealed histopathological changes in liver and kidney as well as its dams. Notably, the nano-silver is considered cytotoxic for HepG2 cell line as well as mouse liver cell line. In conclusions, the Ag-NPs considered toxic in offspring as well as dams and had immunosuppressive effects in the postnatal model of toxicity as well as cytotoxicity to hepatic cells lines. Material Science Research India www.materialsciencejournal.org ISSN: 0973-3469, Vol.17, No.(1) 2020, Pg. 54-61 CONTACT Mahmoud M. Elalfy mahmoudelalfy@mans.edu.eg Forensic and toxicology department, faculty of veterinary medicine, Mansoura University, Egypt. © 2020 The Author(s). Published by Oriental Scientific Publishing Company This is an Open Access article licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License Doi: http://dx.doi.org/10.13005/msri/170108 Article History Received: 01 April 2020 Accepted: 30 April 2020