“Plug and Play”-expression systems for high-quality production of recombinant proteins for structural analysis

Abstract

Determining the structures of proteins of entire proteomes is a formidable endeavour. High throughput structural genomics correspondingly requires the development of highly efficient methods to streamline every step in progressing from the individual gene expression to refined three-dimensional protein structure. A major bottleneck in structural biology is the production of sufficient amounts of correctly folded and functional protein. Here, we present a simple “Plug and Play”-expression system that allows for parallel gene expression in E. coli, yeast and insect cells to rapidly select the best strategy for the production of high-quality protein.