BIOGENIC SYNTHESIS, CHARACTERIZATION OF SILVER NANOPARTICLES FROM CARISSA CARANDAS LEAF EXTRACT AND ITS PHARMACOLOGICAL ACTIVITIES

Abstract

A Plant-mediated synthesis of nanoparticles is considered as a representative approach to environment benignity in material synthesis to avoid chemical toxicity. In this present study, we report an eco-friendly technique for the preparation of silver nanoparticles (AgNPs) from aqueous leaves extract of Carissa carandas Linn, and the evaluation of their pharmacological activities. The formation of AgNPs is confirmed by UV-Visible spectrum which peak highest at 436nm that corresponds to surface plasmon resonance of AgNPs. The shape and size of synthesized AgNPs were determined by HR-TEM and FEG-SEM which indicated well-dispersed nanoparticles with size ranging from 20nm-40nm. The functional groups responsible for reduction of silver ions are demonstrated by FTIR. XRD reported the crystalline nature of AgNPs. DPPH assay shows AgNPs has better antioxidant potential than its aqueous leaves extract. Antibacterial activity of AgNPs using resazurin dye against gram-positive and gram-negative bacteria with minimum inhibition concentrations (MICs) was found to be in the range from 31.5 μg/mL to 125 μg/mL. This biogenic synthesis provides an environment friendly, clean, cost-effective, and easy method for synthesis of AgNPs with enhanced pharmacological activities.